MABF3046

Anti-Mayaro Virus E2 Antibody, clone MAY-134

• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: MAYV-134, monoclonal
• Poids mol.: calculated mol wt 137.06 kDa
• Produit purifié par: using protein G
• Espèces réactives: virus
• Conditionnement: antibody small pack of 100 μL
• Technique(s): ELISA: suitable; neutralization: suitable
• Isotype: IgG2a
• Séquence de l'épitope: Extracellular domain
• Numéro d'accès UniProt: DQ487410
• Conditions d'expédition: dry ice
• Modification post-traductionnelle de la cible: unmodified

Description

Description générale

Structural polyprotein (UniProt: Q8QZ72; also known as p130) is encoded by the MAYVgp3 gene (Gene ID: 935141) in Mayaro virus. Following its synthesis it is cleaved into the following six chains: Capsid protein (aa 1-258), Precursor of protein E3/E2 (aa 259-746), Assembly protein E3 (aa 259-324), Spike glycoprotein E2 (aa 325-746), 6K protein (aa 747-806), and Spike glycoprotein E1 (aa 807-1242). Mayaro virus (MAYV) is a mosquito-transmitted arthritogenic alphavirus in the Togaviridae family of positive-stranded RNA viruses. Its envelope glycoproteins associate in the endoplasmic reticulum and form a heterotrimer comprised of the E3, E2, and E1 proteins. E2 is a single-pass type I membrane protein that plays a role in viral attachment to target host cell, by binding to the cell receptor. It is synthesized as a p62 precursor (precursor of protein E3/E2), which is then processed by furin at the cell membrane just before virion budding, giving rise to E2-E1 heterodimer. The p62-E1 heterodimer is shown to be stable, whereas E2-E1 is unstable and dissociates at low pH. It has been reported that p62 is processed at the last step, presumably to avoid E1 fusion activation before its final export to cell surface. The C-terminus region of E2 contains a transitory transmembrane region that is disrupted by palmitoylation and results in reorientation of the C-terminal tail from lumenal to cytoplasmic side. Clone MAY-134 offers protection against lethal effects of MAYV in a murine model when injected one day prior to virus inoculation. However, its protective effects are significantly reduced when injected two days post infection. It also promotes phagocytosis of MAYV antigen-coated beads in murine neutrophils and monocytes. This clone displays higher protective activity as IgG2a than IgG1 even though their neutralizing activity in vitro and half-lives in vivo were identical. (Ref.: Earnest, JT., et al. (2019). J. Exp. Med. 216(10); 2282-2301).

Spécificité

Clone MAY-134 is a mouse monoclonal antibody that detects E2 protein of Mayaro virus. It targets an epitope within the B domain in the extracellular region.

Immunogène

MAYV strain CH and boosted twice with recombinant, bacterially produced MAYV E2 ectodomain (amino acids 1-340) in Freund s incomplete adjuvant.

Application

Quality Control Testing

Evaluated by ELISA with recombinant Mayaro virus E2 protein.

ELISA Analysis (ELISA): Various dilutions of this antibody detected recombinant E2 protein of Mayaro virus CH (strain Peru).

Tested Applications

Neutralizing: A representative lot neutralized Mayaro Virus E2 in Neutralizing applications (Earnest, J.T., et al. (2019). J Exp Med. 216(10): 2282-2301).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Mayaro Virus E2, clone MAYV-134 , Cat. No. MABF3046, is a mouse monoclonal antibody that detects E2 protein of Mayaro virus CH and is tested for use in ELISA and Neutralizing applications.

Forme physique

Purified mouse monoclonal antibody IgG2a in PBS without azide.

Stockage et stabilité

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable