MABE453
Anti-monoubiquityl Histone H2B (Lys119) Antibody, clone 7B4
clone 7B4, from mouse
Synonyme(s) :
H2BUb1, Histone H2B type 1-C/E/F/G/I, Histone H2B.1 A, Histone H2B.a, H2B/a, Histone H2B.g, H2B/g, Histone H2B.h, H2B/h, Histone H2B.k, H2B/k, Histone H2B.l, H2B/l, H2BUbK119, H2BK119Ub
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
7B4, monoclonal
Espèces réactives
human
Technique(s)
immunohistochemistry: suitable
western blot: suitable
Isotype
IgG2aκ
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... HIST1H2BC(8347)
Description générale
H2BUb1, also known as Histone H2B.1 A, or Histone H2B.a (H2B/a), or Histone H2B.g (H2B/g), or Histone H2B.h (H2B/h), or Histone H2B.k (H2B/k) or Histone H2B.i (H2B/I;H2B/I), and encoded by the gene HIST1H2BC or HIST1H2BE, HIST1H2BF, HIST1H2BG, HIST1H2BI/H2BFL, H2BFH, H2BFG, H2BFA, H2BFK, is a core component of the nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. H2BUb1 also has broad antibacterial activity and may contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid. H2BUb1 is localized to the nucleus and chromosomes in healthy cells.
Immunogène
Ovalbumin-conjugated corresponding to human monoubiquityl Histone H2B (Lys119).
Application
Detect Histone H2B using this mouse monoclonal antibody, Anti-monoubiquityl Histone H2B (Lys119) Antibody, clone 7B4 validated for use in western blotting & IHC.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Western Blotting Analysis: A representative lot detected monoubiquityl Histone H2B (Lys119) in hormone deprived MCF7 cell lysates, which were pretreated with vehicle (ethanol) or Bortezomid before incubating with 17Beta-Estradiol (Prenzel, T., et al. (2011). AACR. OF1-OF15).
Western Blotting Analysis: A representative lot detected monoubiquityl Histone H2B (Lys119) in differentiated hMSCs that were truncated with control and demostrated a loss of signal in differntiated hMSCs that ere transfected with RNF50 siRNA (Karpiuk, O., et al. (2012). Molecular Cell. 46:705-713).
Immunohistochemistry Analysis: A representative lot detected monoubiquityl Histone H2B (Lys119) in malignant, metastatic breast tumor and normal adjacent tissues (Prenzel, T., et al. (2011). AACR. OF1-OF15).
Western Blotting Analysis: A representative lot detected monoubiquityl Histone H2B (Lys119) in differentiated hMSCs that were truncated with control and demostrated a loss of signal in differntiated hMSCs that ere transfected with RNF50 siRNA (Karpiuk, O., et al. (2012). Molecular Cell. 46:705-713).
Immunohistochemistry Analysis: A representative lot detected monoubiquityl Histone H2B (Lys119) in malignant, metastatic breast tumor and normal adjacent tissues (Prenzel, T., et al. (2011). AACR. OF1-OF15).
Qualité
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected monoubiquityl Histone H2B (Lys119) in HeLa cell lysates treated with and without Nocodazole.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected monoubiquityl Histone H2B (Lys119) in HeLa cell lysates treated with and without Nocodazole.
Description de la cible
~20 kDa observed
Forme physique
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Stockage et stabilité
Stable for 1 year at 2-8°C from date of receipt.
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Keqiang Zhang et al.
International journal of cancer, 141(4), 766-777 (2017-05-10)
Deregulated monoubiquitination of histone H2B (H2Bub1), mainly catalyzed by E3 ubiquitin-protein ligase RNF20/RNF40 complex, may play an important role in cancer. Here we investigate potential roles of H2Bub1 and the underlying mechanisms through which it contributes to cancer development and
Mei-Jun Zhao et al.
Oncotarget, 7(8), 8956-8967 (2016-01-21)
The ubiquitin ligase RNF8 promotes the DNA damage response (DDR). We observed that the expression of RNF8 was increased in bladder cancer cells and that this change in RNF8 expression could be reversed by adenovirus-mediated shRNA treatment. Moreover, we found
Keqiang Zhang et al.
Cell communication and signaling : CCS, 17(1), 167-167 (2019-12-18)
Loss of monoubiquitination of histone H2B (H2Bub1) was found to be associated with poor differentiation, cancer stemness, and enhanced malignancy of non-small cell lung cancer (NSCLC). Herein, we investigated the biological significance and therapeutic implications of ubiquitin-specific protease 22 (USP22)
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