MABC942
Anti-PLA2R1 Antibody
mouse monoclonal, 5F5.1
Synonyme(s) :
PLA2-R, PLA2R, Secretory phospholipase A2 receptor, 180 kDa secretory phospholipase A2 receptor, C-type lectin domain family 13 member C, M-type receptor
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
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product name
Anti-PLA2R Antibody, clone 5F5.1, clone 5F5.1, from mouse
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
5F5.1, monoclonal
Espèces réactives
human
Technique(s)
immunohistochemistry: suitable
western blot: suitable
Isotype
IgG1κ
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... PLA2R1(22925)
Description générale
Secretory phospholipase A2 receptor (UniProt Q13018; also known as 180 kDa secretory phospholipase A2 receptor, C-type lectin domain family 13 member C, M-type receptor, Phospholipase A2 receptor 1) is encoded by the PLA2R1 (also known as CLEC13C, PLA2G1R, PLA2R, PLA2IR) gene (Gene ID 22925) in human. PLA2R is initially produced with a signal peptide sequence (a.a. 1-20), the cleavage of which yields the mature type I transmembrane receptor composed of a short cytoplasmic tail (a.a. 1419-1463), a transmembrane segment (a.a. 1419-1463), and a large extracellular portion consisting of an N-terminal cystein-rich region, a fibronectin-like type II domain, and a tandem repeat of eight carbohydrate-recognition domains (CRDs). Both group IB and X secretory phospholipase A2 (sPLA2) enzymes are identified as ligands orf PLA2R, while group IIA sPLA2s bind PLA2R with about 10-fold lower affinity when compared with group IB and X sPLA2s. In mice, the extracellular portion of PLA2R is reported to be cleaved off into a soluble form (sPLA2R) in blood, where it functions as a circulating sPLA2 inhibitory protein (PLI) that blocks both the receptor-binding and enzymatic activity of sPLA2.
Spécificité
Expected to react with both spliced isoforms, as well as the soluble receptor (sPLA2R) derived from metalloproteinases-mediated cleavage of the membrane-bound receptor.
Immunogène
Epitope: Extracellular C-type lectin domains 3-5
GST-tagged recombinant protein corresponding to the extracellular C-type lectin domains 3-5 of human PLA2R.
Application
This Anti-PLA2R Antibody, clone 5F5.1 is validated for use in Western Blotting and Immunohistochemistry for the detection of PLA2R.
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected PLA2R in 10 µg of human kidney tissue lysate.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected PLA2R in human kidney tissue.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected PLA2R in human kidney tissue.
Qualité
Evaluated by Western Blotting in human lung tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected PLA2R in 10 µg of human lung tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected PLA2R in 10 µg of human lung tissue lysate.
Description de la cible
~180 kDa observed. Target band(s) appears larger than the calculated molecular weights (166.6 kDa/isoform 1 and 150.8 kDa/isoform 2) due to posttranslational glycosylation. Uncharacterized band(s) may appear in some lysates.
Forme physique
Format: Purified
Autres remarques
Concentration: Please refer to lot specific datasheet.
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Réf. du produit
Description
Tarif
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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