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Key Documents

MABC008

Sigma-Aldrich

Mouse IgM Negative Control, clone GC323

Mouse IgM Negative Control Monoclonal Antibody validated for use in Flow Cytometry & Immunofluorescence.

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.42

Source biologique

mouse

Niveau de qualité

Clone

GC323, monoclonal

Fabricant/nom de marque

Chemicon®

Concentration

0.5 mg/mL

Technique(s)

flow cytometry: suitable
immunofluorescence: suitable

Isotype

IgM

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Spécificité

This antibody reacts specifically with the outer membrane protein I component of various strains of Neisseria gonorrhoeae and does not react with any human cell surface component.

Immunogène

Protein I preparation of Neisseria gonorrhoeae.

Application

Research Category
Secondary & Control Antibodies

Epitope Tags & General Use
Research Sub Category
Isotype Control Antibodies
The use of this antibody enables an estimation of non-specific binding of mouse monoclonal antibodies of isotype IgM to cell surface components in human blood and bone marrow. It is not suitable for use in immunohistochemical procedures.

SUGGESTED USAGE

The control antibody should be diluted to the same concentration as the test antibody, and equivalent volumes used. Flow cytometry and immunofluorescence - use 10 μl direct from the vial per 100 μl of whole blood, or 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.

Forme physique

Format: Purified
Purified from mouse ascitic fluid. The antibody is supplied in 0.5ml 25mM Tris HCl, 0.4M NaCl, pH 8.0, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by electrophoresis, specific immunofluorescence assay and flow cytometry.

Stockage et stabilité

Store at 2 to 8°C, for up to 6 months. For prolonged periods, store below -20°C in undiluted aliquots. AVOID REPEATED FREEZE/THAW CYCLES.

WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictogrammes

Corrosion

Mention d'avertissement

Warning

Mentions de danger

Conseils de prudence

Classification des risques

Met. Corr. 1

Code de la classe de stockage

8B - Non-combustible corrosive hazardous materials

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Chih-Jen Lin et al.
PloS one, 9(9), e106916-e106916 (2014-09-12)
The ability of small molecules to maintain self-renewal and to inhibit differentiation of pluripotent stem cells has been well-demonstrated. Two widely used molecules are PD 98059 (PD), an inhibitor of extracellular-signal-regulated kinase 1 (ERK), and SC1 (Pluripotin), which inhibits the
Acceptance of embryonic stem cells by a wide developmental range of mouse tetraploid embryos.
Lin, CJ; Amano, T; Zhang, J; Chen, YE; Tian, XC
Biology of Reproduction null
Mari Kamiya et al.
Nature communications, 13(1), 166-166 (2022-01-12)
Muscle cell death in polymyositis is induced by CD8+ cytotoxic T lymphocytes. We hypothesized that the injured muscle fibers release pro-inflammatory molecules, which would further accelerate CD8+ cytotoxic T lymphocytes-induced muscle injury, and inhibition of the cell death of muscle
Shirley Lam et al.
mAbs, 7(6), 1178-1194 (2015-08-26)
Chikungunya virus (CHIKV) is a medically important human viral pathogen that causes Chikungunya fever accompanied with debilitating and persistent joint pain. Host-elicited or passively-transferred monoclonal antibodies (mAb) are essential mediators of CHIKV clearance. Therefore, this study aimed to generate and

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