MAB1951Z
Anti-Integrin β1 Antibody, clone P4G11, azide free
clone P4G11, Chemicon®, from mouse
Synonyme(s) :
CD29, MAB1951
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
P4G11, monoclonal
Espèces réactives
monkey, human, rabbit
Fabricant/nom de marque
Chemicon®
Technique(s)
ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
Isotype
IgG1
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... ITGB1(3688)
Spécificité
Human integrin beta1. The epitope recognized by MAB1951Z is Ca2+ dependent.
Immunogène
Human IL-1beta activated endothelial cells
Application
Immunohistochemistry: for use on acetone or mild paraformaldehyde fixed tissue (2-2.5% PFA, short time) frozen and 4% PFA paraffin embedded {Qin et al 2003 1:500, citrate HEIR treated}
Ca++ required for binding. Ca++ must be used in blocking, washing, and antibody incubation buffers.
Traditional formalin fixation is not recommended.
Immunocytochemistry: 2 ug/mL (for use on numerous cell lines as long as Ca2+ is present)
Immunoprecipitation: 5 μg/400 μL lysate (0.5% triton PBS plus Ca++)
FACS: 2-5μg per 10e6 cells, in the presence of Ca++.
Stimulation of cells: 2-10 μg/ml per 10e6 cells. Binding buffer must contain 1mM Ca++.
ELISA
Optimal working dilutions must be determined by end user.
Ca++ required for binding. Ca++ must be used in blocking, washing, and antibody incubation buffers.
Traditional formalin fixation is not recommended.
Immunocytochemistry: 2 ug/mL (for use on numerous cell lines as long as Ca2+ is present)
Immunoprecipitation: 5 μg/400 μL lysate (0.5% triton PBS plus Ca++)
FACS: 2-5μg per 10e6 cells, in the presence of Ca++.
Stimulation of cells: 2-10 μg/ml per 10e6 cells. Binding buffer must contain 1mM Ca++.
ELISA
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Integrins
Integrins
This Anti-Integrin β1 Antibody, clone P4G11, azide free is validated for use in ELISA, FC, IP, FUNC, FUNC, IC, IH for the detection of Integrin β1.
Forme physique
Format: Purified
Sterile-filtered protein A purified immunoglobulin in 0.01M PBS, pH 7.1, containing no preservatives.
Stockage et stabilité
Maintain between 2 and 8°C for up to 6 months from date of receipt. Avoid freeze thaws.
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Vous ne trouvez pas le bon produit ?
Essayez notre Outil de sélection de produits.
En option
Réf. du produit
Description
Tarif
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Garima Dwivedi et al.
Cartilage, 9(4), 378-390 (2017-11-22)
Bone marrow stimulation procedures initiate repair by fracturing or drilling subchondral bone at base of cartilaginous defect. Earlier studies have shown that defect location and animal age affect cartilage repair outcome, suggesting a strong influence of structural and biological characteristics
Hsin-Ling Yin et al.
International journal of molecular sciences, 17(9) (2016-09-03)
Triple negative breast cancer (TNBC) displays higher risk of recurrence and distant metastasis. Due to absence of estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor 2 (HER2), TNBC lacks clinically established targeted therapies. Therefore, understanding of
Todd J Herron et al.
Circulation. Arrhythmia and electrophysiology, 9(4), e003638-e003638 (2016-04-14)
Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) monolayers generated to date display an immature embryonic-like functional and structural phenotype that limits their utility for research and cardiac regeneration. In particular, the electrophysiological function of hPSC-CM monolayers and bioengineered constructs used to
Garima Dwivedi et al.
Cartilage, 12(2), 237-250 (2018-12-21)
Previous studies have shown that intrinsic behavior of subchondral bone marrow stem cells (BMSCs) is influenced by donors and locations. To understand the variability in cartilage repair outcomes following bone marrow stimulation, we tested the hypothesis that in vivo cartilage
Stephen P Evanko et al.
Matrix biology : journal of the International Society for Matrix Biology, 42, 74-92 (2015-01-01)
The contribution of hyaluronan-dependent pericellular matrix to TGF-β1-driven induction and maintenance of myofibroblasts is not understood. Hyaluronan is an extracellular matrix (ECM) glycosaminoglycan important in cell adhesion, proliferation and migration, and is implicated in myofibroblast formation and maintenance. Reduced turnover
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique