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MAB1096

Sigma-Aldrich

Anti-Tumor Necrosis Factor-α Antibody, clone 195

clone 195, Chemicon®, from mouse

Synonyme(s) :

TNF-alpha

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

100

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

195, monoclonal

Espèces réactives

human

Ne doit pas réagir avec

pig, monkey, canine, mouse, rat, baboon

Conditionnement

antibody small pack of 25 μg

Fabricant/nom de marque

Chemicon®

Technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
neutralization: suitable
western blot: suitable

Isotype

IgG3

Numéro d'accès NCBI

NM_000594.2

Numéro d'accès UniProt

P01375

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... TNF(7124)

Spécificité

The antibody reacts with human, natural and recombinant TNF-alpha. lt does not react with human TNF-beta (lymphotoxin). lt does not react with TNF-alpha from mouse, rat, canine, porcine, rhesus-monkey or baboon.

SPECIES REACTIVITIES:

Not mouse, rat, canine, porcine, baboon or rhesus-monkey.

Immunogène

Human, recombinant tumor necrosis factor-alpha produced in E. coli.

Application

Research Category
Signaling
Research Sub Category
Growth Factors & Receptors
This Anti-Tumor Necrosis Factor-α Antibody, clone 195 is validated for use in IC, IH, NEUT, WB for the detection of Tumor Necrosis Factor-α.
Western Blot

Immunocytochemistry

Immunohistochemistry

Neutralization: 50 ng of TNF-alpha antibody is required for the neutralization of 1 U TNF-alpha. The TNF-alpha-preparation which is used has a specific activity of 2 x 10(7) U/mg protein [cell lytic assay with L929 cells (mouse transformed fibroblasts) and/or WEHJ 164 cells (mouse fibrosarcoma cells)/actinomycin D].

Optimal working dilutions must be determined by end user.

Liaison

Replaces: 04-1114

Forme physique

Format: Purified
Purified immunoglobulin. Liquid in 0.01M PBS pH 7.1, 0.15M NaCl containing no preservatives. Sterile filtered.

Stockage et stabilité

Maintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. Freeze only once.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Micaela Morgado et al.
Oncotarget, 7(12), 14871-14884 (2016-02-27)
Transmembrane mucins (TMs) are restricted to the apical surface of normal epithelia. In cancer, TMs not only are over-expressed, but also lose polarized distribution. MUC16/CA125 is a high molecular weight TM carrying the CA125 epitope, a well-known molecular marker for
Mei Liu et al.
International journal of oncology, 38(4), 1013-1022 (2011-01-29)
Many studies have demonstrated the overexpression and amplification of the miR-17-92 cluster in malignant human cancers, including B-cell lymphomas and lung cancers. The purpose of this study was to investigate for the first time, the expression of the miR-17-92 cluster
Emanuele Garzia et al.
Fertility and sterility, 100(1), 108-115 (2013-04-02)
To investigate the expression of prolactin (PRL), PRL-receptor (PRL-R), and the TH1 cytokines interleukin-2 (IL-2), tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ) at the maternofetal interface. Case-control study. University hospital unit of gynecology and obstetrics and research laboratories. Women undergoing

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