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DC03L

Sigma-Aldrich

Goat Anti-Rabbit IgG Peroxidase Conjugate

lyophilized, Calbiochem®

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

goat

Niveau de qualité

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

secondary antibodies

Clone

polyclonal

Forme

lyophilized

Ne contient pas

preservative

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

OK to freeze

Conditions d'expédition

ambient

Température de stockage

2-8°C

Modification post-traductionnelle de la cible

unmodified

Description générale

Immunoaffinity purified goat polyclonal antibody adsorbed against human serum proteins and conjugated to horseradish peroxidase. Recognizes rabbit IgG.
This Goat Anti-Rabbit IgG Peroxidase Conjugate is validated for use in ELISA, Immunohistochemistry for the detection of Rabbit IgG.

Application

ELISA (1:5000-1:100,000)

Immunoblotting (1:5000-1:100,000, colorimetric; 1:10,000-1:200,000, chemiluminescence)

Immunohistochemistry (1:500-1:5000)

Avertissement

Toxicity: Standard Handling (A)

Forme physique

Lyophilized from 250 mM NaCl, 10 mM sodium phosphate, 15 mg/ml BSA, pH 7.6.

Reconstitution

Reconstitute the antibody with 1.5 ml H₂O. Dissolve by gently mixing at 4°C, since extensive mixing will denature antibodies. Following reconstitution, aliquot and freeze (-70°C). Avoid freeze/thaw cycles of solutions. Alternatively, glycerol (to a final concentration of 50%) may be added and the reconstituted antibody then stored at -20°C. Please note, under these conditions the concentration of the antibody and the buffer salts will be one-half of the original concentration.

Autres remarques

To ensure the quality of these reagents each lot is tested with several of our primary antibodies. Antibody specificity is tested by immunoelectrophoresis and shown to be specific for rabbit IgG and light chains common to most rabbit immunoglobulins. No cross-reactivity with non-immunoglobulin serum proteins has been detected. The antibody has also been tested by ELISA and/or adsorbed to ensure minimal cross-reactivity with human serum proteins, but the antibody may exhibit cross-reactivity with immunoglobulins from other species. Antibody should be titrated for optimal results in individual systems.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3


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Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Karl A Nath et al.
The American journal of pathology, 166(4), 963-972 (2005-03-29)
Ischemic injury is invoked as a mechanism contributing to end-organ damage and other complications of sickle cell disease (SCD). However, the intrinsic sensitivity of tissues in SCD to ischemic insults has never been addressed. We examined the effect of renal
Katherine A Blackwell et al.
American journal of physiology. Heart and circulatory physiology, 287(6), H2448-H2453 (2004-08-21)
Oxidative stress has been implicated as an important mechanism of vascular endothelial dysfunction induced by aging. Previous studies suggested that tetrahydrobiopterin (BH4), an essential cofactor of endothelial NO synthase, could be a molecular target for oxidation. We tested the hypothesis
Emmanuelle Thinon et al.
Journal of proteome research, 17(5), 1907-1922 (2018-03-27)
S-Fatty-acylation is the covalent attachment of long chain fatty acids, predominately palmitate (C16:0, S-palmitoylation), to cysteine (Cys) residues via a thioester linkage on proteins. This post-translational and reversible lipid modification regulates protein function and localization in eukaryotes and is important
K Tello et al.
Radiation and environmental biophysics, 47(3), 389-397 (2008-05-22)
In a previous publication, we were able to show that irradiation of Kupffer cells, the liver resident macrophages, leads to an increased TNF-alpha concentration in the culture medium. The pathomechanisms underlying this phenomenon, however, remained to be elucidated. Here, we
Bettina P Mihalas et al.
Aging, 11(10), 2968-2997 (2019-05-28)
Oocytes are reliant on messenger RNA (mRNA) stores to support their survival and integrity during a protracted period of transcriptional dormancy as they await ovulation. Oocytes are, however, known to experience an age-associated alteration in mRNA transcript abundance, a phenomenon

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