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AQ502P

Sigma-Aldrich

Goat Anti-Mouse IgM+IgG+IgA Antibody, F(ab′)2, HRP conjugate, Species Adsorbed

Chemicon®, from goat

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.46

Source biologique

goat

Niveau de qualité

Conjugué

peroxidase conjugate

Forme d'anticorps

purified antibody

Type de produit anticorps

secondary antibodies

Clone

polyclonal

Espèces réactives

mouse

Fabricant/nom de marque

Chemicon®

Technique(s)

ELISA: suitable

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Description générale

IgG is the most abundant immunoglobulin and comprises two heavy chains and two light chains. Each molecule has two antigen binding sites. IgG has 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). IgM constitutes about 10% of serum immunoglobulins. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces.

Spécificité

Reacts with heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA. Absorbed for human sera and purified human paraproteins. Minimal cross reactivity with human immunoglobulins.
The antibody reacts with the heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with human immunoglobulins.

Immunogène

Epitope: Mouse IgM, IgG, IgA
Prepared from pepsin digest of mouse Ig (H+L).

Application

ELISA: 1:4,000-1:8,000.

Optimal working dilutions must be determined by the end user.
Research Category
Secondary & Control Antibodies
Research Sub Category
Fragment Specific Secondary Antibodies
This Goat anti-Mouse IgM+IgG+IgA Antibody, F(ab′)2, HRP conjugate, Species Adsorbed is validated for use in ELISA for the detection of Mouse IgM+IgG+IgA.

Forme physique

Gel filtration chromatography
Purified by gel filtration chromatography of pepsin digested antibody. Liquid in 50% glycerol/50% PBS, pH 7.4.

Stockage et stabilité

Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt. For long term storage, aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2


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Consulter la Bibliothèque de documents

Yuliang Wu et al.
Nature protocols, 2(12), 3278-3284 (2007-12-15)
Far western blotting (WB) was derived from the standard WB method to detect protein-protein interactions in vitro. In Far WB, proteins in a cell lysate containing prey proteins are firstly separated by SDS or native PAGE, and transferred to a
John E La Marca et al.
International journal of molecular sciences, 22(23) (2021-12-11)
Tissue homeostasis via the elimination of aberrant cells is fundamental for organism survival. Cell competition is a key homeostatic mechanism, contributing to the recognition and elimination of aberrant cells, preventing their malignant progression and the development of tumors. Here, using

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