ABN899

Anti-Olig-2 Antibody

from rabbit, purified by affinity chromatography

• Synonyme(s) : Oligodendrocyte transcription factor 2, bHLHe19, Olg-2, Oligo2, RACK17, RK17
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: mouse, rat, human
• Technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• Numéro d'accès NCBI: NP_058663
• Numéro d'accès UniProt: Q9EQW6
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... OLIG2(10215)

Description

Description générale

Oligodendrocyte transcription factor 2 (UniProt Q9EQW6; also known as bHLHe19, Olg-2, Oligo2, RACK17, RK17) is encoded by the Olig2 (also known as Bhlhb1) gene (Gene ID 50913) in murine species. Olig2 is a basic helix-loop-helix (bHLH) transcription factor expressed in a spinal cord ventricular zone domain where it mediates the sequential generation of motoneurons and oligodendrocytes during embryogenesis. Olig2 also functions as an anti-neurogenic factor in pMN progenitors at early time points to sustain the cycling progenitor pool. The anti-neurogenicity of Olig2 is also responsible for its role in tumorigenesis, especially in brain tumors and leukemia. Due to its expression in glioblastoma and other diffuse gliomas, Olig2 is a useful diagnostic marker of these brain tumors.

Immunogène

KLH-conjugated linear peptide corresponding to a sequence in the N-terminal region of mouse Olig-2.

Application

Anti-Olig-2 Antibody is an antibody against Olig-2 for use in Western Blotting, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry (Paraffin).

Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated Olig-2 in 500 µg of mouse brain tissue lysate.
Immunohistochemistry Analysis: An 1:500 dilution from a representative lot detected Olig-2 in rat hindbrain tissue sections.
Immunocytochemistry Analysis: An 1:500 dilution from a representative lot detected Olig-2 in PC12 cells.

Qualité

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Olig-2 in 10 µg of mouse brain tissue lysate.

Description de la cible

~37 kDa observed. 32.40 kDa calculated.

Liaison

Replaces: AB15328

Forme physique

Purified rabbit polyclonal antibody in PBS and 0.1 % sodium azide.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable