ABN458
Anti-Choline Transporter Antibody
from rabbit, purified by affinity chromatography
Synonyme(s) :
High affinity choline transporter 1, Hemicholinium-3-sensitive choline transporter, CHT, Solute carrier family 5 member 7
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Produit purifié par
affinity chromatography
Espèces réactives
human, rat, mouse
Technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... SLC5A7(60482)
Description générale
The protein named Choline Transporter or Hemicholinium-3-sensitive choline transporter (CHT), and encoded by the gene SLC5A7/CHT1, is the high affinity choline transporter in neurons. Choline is the rate limiting step in acetylcholine synthesis an important neurotransmitter. Acetylcholine is a neurotransmitter of the central and peripheral nervous system that regulates a variety of autonomic, cognitive, and motor functions. The choline transporter is localized to the cell membrane and is expressed in cholinergic neurons particularly in the putamen, spinal cord and medulla. Defects in the choline transporter may be associated with a syndrome named distal spinal muscular atrophy with vocal cord paralysis. The choline transporter protein is a Na+ and Cl- dependent transporter that can be inhibited by nanomolar concentrations of the chemical compound hemicholinium 3, a compound often used to deplete acetylcholine stores.
Immunogène
GST-tagged recombinant protein corresponding to the C-terminus of human Choline Transporter.
Application
Anti-Choline Transporter Antibody is a highly specific rabbit polyclonal antibody, that targets Choline Transporter & has been tested in western blotting & IHC (Paraffin).
Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Choline Transporter in rat spinal cord and human cerebral cortex tissue.
Qualité
Evaluated by Western Blotting in rat spinal cord tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Choline Transporter in 10 µg of rat spinal cord tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Choline Transporter in 10 µg of rat spinal cord tissue lysate.
Description de la cible
~70-75 kDa observed. Uniprot states MW at ~63kDa but lists one glycosylation site which adds ~5kDa to the total MW.
Liaison
Replaces: AB5960;AB5966
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Ajeesh Koshy Cherian et al.
Neurochemistry international, 108, 410-416 (2017-06-05)
The synaptic uptake of choline via the high-affinity, hemicholinium-3-dependent choline transporter (CHT) strongly influences the capacity of cholinergic neurons to sustain acetylcholine (ACh) synthesis and release. To advance research on the impact of CHT capacity in humans, we established the
Ajeesh Koshy Cherian et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 37(11), 2947-2959 (2017-02-15)
Some rats [sign-trackers (STs)] are prone to attribute incentive salience to reward cues, which can manifest as a propensity to approach and contact pavlovian cues, and for addiction-like behavior. STs also exhibit poor attentional performance, relative to goal-trackers (GTs), which
Eryn Donovan et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 42(16), 3426-3444 (2022-03-03)
Transport of choline via the neuronal high-affinity choline transporter (CHT; SLC5A7) is essential for cholinergic terminals to synthesize and release acetylcholine (ACh). In humans, we previously demonstrated an association between a common CHT coding substitution (rs1013940; Ile89Val) and reduced attentional
Maximilian I Pinkham et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 312(3), R368-R379 (2017-01-06)
Heart failure is characterized by the loss of sympathetic innervation to the ventricles, contributing to impaired cardiac function and arrhythmogenesis. We hypothesized that renal denervation (RDx) would reverse this loss. Male Wistar rats underwent myocardial infarction (MI) or sham surgery
Yoshiko Murakata et al.
Frontiers in cardiovascular medicine, 9, 1075358-1075358 (2023-02-07)
There is growing evidence indicating a close relationship between inflammation and atrial fibrillation (AF). Although underlying inflammatory atrial cardiomyopathy may contribute to the development of AF, the arrhythmogenic remodeling caused by atrial inflammation has not been elucidated in detail. Herein
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