ABN426

Anti-phospho-Neurogranin (Ser36)/Neuromodulin (Ser41) Antibody

serum, from rabbit

• Synonyme(s) : Protein kinase C substrate 7.5 kDa protein RC3
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: serum
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Espèces réactives: mouse
• Réactivité de l'espèce (prédite par homologie): rat (based on 100% sequence homology)
• Technique(s): western blot: suitable
• Numéro d'accès NCBI: NP_077054
• Numéro d'accès UniProt: Q04940
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: phosphorylation (pSer36)
• Informations sur le gène: mouse ... Nrgn(64011) ; rat ... Nrgn(64356)

Description

Description générale

Neurogranin (Ng) (also named RC3, p17 or BICKS) is a small protein originally identified in rat brain and abundantly expressed in several telencephalic areas, such as the cerebral cortex, hippocampus, amygdala, and striatum. In neurons, it is found concentrated at dendritic spines where it participates in synaptic signaling events through the regulation of calmodulin (CaM). Neurogranin protein is a critical third messenger and substrate of PKC in the molecular cascade necessary for synaptic development and remodeling. Neurogranin (Ng) features an IQ motif that mediates its interaction with CaM and phosphatidic acid (PA). The interaction is controlled by phosphorylation at serine 36 of Neurogranin. Neurogranin is phosphorylated at serine 36 by PKC. Ser36-phosphorylated Neurogranin is unable to bind either CaM or PA. Neurogranin in localized in the cell bodies of neurons in the cortex and in the apical and basal dendrites of pyramidal neurons. Neurogranin is not found in dendrites and its expression is very low as well in Alzheimer’s disease patients.

Spécificité

Phosphorylated Neurogranin and Neuromodulin

Immunogène

Epitope: IQ domain

KLH-conjugated linear peptide corresponding to the IQ domain of Rat phospho-Neurogranin.

Application

Research Category
Neuroscience

Research Sub Category
Neuroregenerative Medicine

This Anti-phospho-Neurogranin (Ser36)/Neuromodulin (Ser41) Antibody is validated for use in Western Blotting for the detection of phospho-Neurogranin (Ser36)/Neuromodulin (Ser41).

Qualité

Evaluated by Western Blotting in Mouse brain tissue lysate.

Western Blotting Analysis: A 1:1000 dilution of this antibody detected phospho-Neurogranin (Ser36) / Neuromodulin (Ser41) in 10 µg of Mouse brain tissue lysate treate with magnisium (lane1) or PKC (lane2)

Description de la cible

~43 kDa is phospho-Neuromodulin (GAP-43) and ~17 KD is phospho-Neurogranin. Uncharacterized band(s) may appear in some lysates.

Forme physique

Rabbit Polyclonal serum with 0.05% sodium azide.

Unpurified

Stockage et stabilité

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable