ABN1640

Anti-Prodynorphin/Leumorphin (dynorphin B29)

serum, from rabbit

• Synonyme(s) : Preproenkephalin-B, Proenkephalin-B, Beta-neoendorphin-dynorphin, Leumorphin, Dynorphin B-29
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: serum
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Espèces réactives: mouse, rat, human
• Technique(s): western blot: suitable
• Numéro d'accès NCBI: NP_062247
• Numéro d'accès UniProt: P06300
• Conditions d'expédition: dry ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... PDYN(5173) ; rat ... Pdyn(29190)

Description

Description générale

Proenkephalin-B (UniProt P06300; also known as Beta-neoendorphin-dynorphin, Preprodynorphin) is encoded by the Pdyn gene (Gene ID 29190) in rat species. Proenkephalin B is an opioid polypeptide hormone involved with chemical signal transduction and cell communication. Prodynorphin is a basic building-block of endorphins, the chemical messengers in the brain that appear most heavily involved in the anticipation and experience of pain and the formation of deep emotional bonds critical in learning and memory. Prodynorphin is produced with a signal peptide (a.a. 1-21) and a propeptide (a.a. 22-163) sequence. Following the removal of the signal and propeptide sequences, the protein is further processed by proeolytic cleavages to form Alpha-neoendorphin (a.a. 166-175), Beta-neoendorphin (a.a. 166-174), Big dynorphin (a.a. 202-233; Big Dyn), Dynorphin A(1-17) (a.a. 202-218; Dyn-A17; Dynorphin A); Dynorphin A(1-13) (a.a. 202-214), Dynorphin A(1-8) (a.a. 202-209), Leu-enkephalin (a.a. 202-206 & 221-225), Leumorphin (Dynorphin B-29; a.a. 221-248), and Rimorphin (a.a. 221-233; Dynorphin B; Dyn-B; Dynorphin B(1-13)).

Spécificité

This polyclonal antiserum recognizes preproenkephalin-B (a.a. 1-248 of rat sequence), proenkephalin-B (a.a. 22-248 of rat sequence), and leumorphin/dynorphin B-29 (a.a. 221-248 of rat sequence), but not other peptides derived from Proenkephalin-B.

Immunogène

Epitope: Leumorphin peptide.

Linear peptide corresponding to rat leumorphin (dynorphin B-29) C-terminal half sequence.

Application

Anti-Prodynorphin Antibody is an antibody against Leumorphin (dynorphin B29) for use in Western Blotting.

Western Blotting Analysis: A 1:500 dilution from a representative lot detected leumorphin (dynorphin B29) in 10 µg of human brain tissue lysate.

Qualité

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected prodynorphin in 10 µg of rat brain tissue lysate.

Description de la cible

~36/3.5 kDa oberved. 28.08/28.06/28.39 kDa (Rat/Mouse/Human preproenkephalin-B), 25.85/25.76/26.28 kDa (Rat/Mouse/Human proenkephalin-B), 3.320/3.320/3.352 kDa (Rat/Mouse/Human leumorphin) calculated. Uncharacterized band(s) may appear in some lysates.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable