ABE1049
Anti-NRF-1 Antibody
serum, from goat
Synonyme(s) :
Nuclear respiratory factor 1, Alpha-pal, Alpha palindromic-binding protein, NRF-1
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
goat
Niveau de qualité
Forme d'anticorps
serum
Type de produit anticorps
primary antibodies
Clone
polyclonal
Espèces réactives
human
Technique(s)
electrophoretic mobility shift assay: suitable
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... NRF1(4899)
Description générale
Nuclear respiratory factor 1 (UniProt Q16656; also known as Alpha-pal, Alpha palindromic-binding protein, NRF-1) is encoded by the NRF1 (Also known as ALPHA-PAL) gene (Gene ID 4899) in human. NRF-1 is a redox-responsive transcription factor that plays an anti-oxidant, pro-survival role in mitochondrial biogenesis process. NRF-1 is upregulated and activated/phosphorylated by Akt in response to oxidant exposure or increased cellular reactive oxygen species (ROS), reulsting in NRF-1 nuclear translocation, where it binds to DNA as a homodimer and mediates the transcription of mitochondrial transcription factor A (mtTFA) that is needed for mitochondrial DNA transcription.
Spécificité
Expected to react with all four spliced isoforms of human NRF-1 reported by UniProt (Q16656). This antiserum bound DNA-NRF-1 complex as evidenced by a specific supershift of NRF-1, but not NRF-2, DNA complex in EMSA (Virbasius, C.A., et al. (1993). Genes Dev. 7(12A):2431-2445).
Immunogène
Recombinant human NRF-1.
Application
Anti-NRF-1 Antibody is an antibody against NRF-1 for use in Western Blotting, Electrophoretic Mobility Shift Assay.
Electrophoretic Mobility Shift Assay (EMSA): A representative lot caused a supershift of DNA-NRF-1 complex in EMSA using radiolabeled oligonucleotide containing human mitochondrial transcription specificity factor genes (TFB1M & TFB2M) NRF-1-binding site and HeLa nuclear extract (NE) or purified recombinant NRF-1 (Gleyzer, N., et al. (2005). Mol. Cell. Biol. 25(4):1354-1366).
Electrophoretic Mobility Shift Assay (EMSA): A representative lot caused a supershift of DNA-NRF-1 complex in EMSA using radiolabeled oligonucleotide containing rat cytochrome C NRF-1-binding site and HeLa nuclear extract (NE), purified HeLa NRF-1 or recombinant NRF-1. No shift by this antiserum of HeLa NE proteins complexed with oligonucleotide containing mouse COXVb gene NRF-2-binding site was observed (Virbasius, C.A., et al. (1993). Genes Dev. 7(12A):2431-2445).
Electrophoretic Mobility Shift Assay (EMSA): A representative lot caused a supershift of DNA-NRF-1 complex in EMSA using radiolabeled oligonucleotide containing rat cytochrome C NRF-1-binding site and HeLa nuclear extract (NE), purified HeLa NRF-1 or recombinant NRF-1. No shift by this antiserum of HeLa NE proteins complexed with oligonucleotide containing mouse COXVb gene NRF-2-binding site was observed (Virbasius, C.A., et al. (1993). Genes Dev. 7(12A):2431-2445).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Qualité
Evaluated by Western Blotting in HeLa nuclear extract.
Western Blotting Analysis: An 1:200 dilution of this antibody detected NRF1 in 10 µg of HeLa nuclear extract.
Western Blotting Analysis: An 1:200 dilution of this antibody detected NRF1 in 10 µg of HeLa nuclear extract.
Description de la cible
~68 kDa observed. 53.54 kDa (isoform Long), 46.16 kDa (isoform Short), 36.05 kDa (isoform 3), 55.67 kDa (isoform 4) calculated.
Forme physique
Goat polyclonal serum with 0.05% sodium azide.
Unpurified
Stockage et stabilité
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Autres remarques
Concentration: Please refer to lot specific datasheet.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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