ABC503

Anti-Cathepsin S

from rabbit, purified by affinity chromatography

• Synonyme(s) : EC: 3.4.22.27
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human
• Technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotype: IgG
• Numéro d'accès NCBI: NP_004070.3
• Numéro d'accès UniProt: P25774
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... CTSS(1520)

Description

Description générale

Cathepsin S (Uniprot: P25774; EC: 3.4.22.27) is encoded by the CTSS gene (Gene ID: 1520) in human. Cathepsin S is a lysosomal thiol protease that removes invariant chain from MHC class II molecules. It displays higher activity on ZVal-val-Arg-/-Xaa compounds and less activity on Z-Phe-Arg-/-NHMec. Cathepsin S is secreted with a signal (aa 1-16) and a pro-peptide (aa 17-114) sequence that are cleaved to produce active enzyme (aa 115-331). Cathepsin S is involved in antigen presentation, general protein catabolism, and tumor progression. Because of its redistribution or increased levels in human and animal tumors, cathepsin S plays a role in invasion and metastasis. Inhibition of cathepsin S is shown to induce autophagy in tumor cells through EGFR signaling pathway by facilitating EGF-mediated EGFR degradation. Cathepsin S inhibition is shown to delay EGFR degradation and cause EGFR accumulation in the late endosomes at the perinuclear region. (Ref.: Beers, C et al. (2005). J. Immunol. 174(3): 1205-1212; Huang CC et al. (2016). Sci. Rep. 6:29256).

Spécificité

This polyclonal antibody detects cathepsin S in human cells. It targets an epitope within 21 amino acids from the internal region.

Immunogène

KLH-conjugated linear peptide corresponding to 21 amino acids from the internal region of human cathepsin S.

Application

Anti-Cathepsin S. Cat. No. ABC503, is a rabbit polyclonal antibody that detects Cathepsin S and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Cathepsin S in human lymphoma, human small intestine, and human pancreas tissues.

Research Category
Apoptosis & Cancer

Qualité

Evaluated by Western Blotting in human testis tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected Cathepsin S in 10 µg of human testis tissue lysate.

Description de la cible

~37 kDa observed; 37.50 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1