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Key Documents

AB3282

Sigma-Aldrich

Anti-Glutathione-S-Transferase Antibody, S. japonicum form

Chemicon®, from rabbit

Synonyme(s) :

GST-tag

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Espèces réactives

Schistosoma japonicum, vertebrates

Fabricant/nom de marque

Chemicon®

Technique(s)

ELISA: suitable
western blot: suitable

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... GSTP1(2950)

Spécificité

Anti-Glutathione S-transferase (GST) is a polyclonal antibody to the recombinant glutathione S-transferase (GST) protein from Schistosoma japonicum.

The anti GST antibody reacts specifically with the glutathione S-transferase moiety of GST fusion proteins as expressed in E.coli by the pGEX (1) vectors and in yeast by the pYEX(TM) vectors (2).


(1)The pGEX expression vector system is a plasmid and purification system which, in most cases, results in the fusion protein being soluble in aqueous solutions for extraction and purification from crude bacterial lysates. This expression vector system enables the protein glutathione S-transferase (GST) to be produced in fusion with the protein of interest so as to bind with high affinity to a glutathione affinity column. The GST-protein complex can then be easily purified by elution from the column with reduced glutathione. The pGEX vectors are available as the GST System from Pharmacia Biotech.

(2) pYEX is a yeast GST expression vector supplied by AMRAD biotech of Australia.

Immunogène

Epitope: S. japonicum form
Recombinant glutathione S-transferase (GST) protein from Schistosoma japonicum.

Application

Anti-Glutathione-S-Transferase Antibody, S. japonicum form is an antibody against Glutathione-S-Transferase for use in ELISA & WB.
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags
The anti-GST antibody can be used to detect GST fusion proteins in Western blot analysis and ELISA studies.

Forme physique

Format: Purified
Liquid in PBS, pH 7.4, containing 0.02% Tween 20 and 0.02% sodium azide as a preservative.

Stockage et stabilité

The anti GST antibody is shipped liquid at 4°C. The anti GST antibody is stable for up to 6 months when stored at 2-8°C.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Carolyn Nicole Brown et al.
iScience, 24(10), 103184-103184 (2021-10-21)
The Ca2+/Calmodulin-dependent protein kinase II (CaMKII) is a central regulator of synaptic plasticity and has been implicated in various neurological conditions, including schizophrenia. Here, we characterize six different CaMKIIα variants found in patients with schizophrenia. Only R396stop disrupted the 12-meric
Zhanguang Zhang et al.
The Journal of experimental medicine, 212(12), 2165-2182 (2015-11-11)
DNAM-1 (CD226) is an activating receptor expressed on natural killer (NK) cells, CD8(+) T cells, and other immune cells. Upon recognition of its ligands, CD155 and CD112, DNAM-1 promotes NK cell-mediated elimination of transformed and virus-infected cells. It also has
Shabarni Gupta et al.
Oncotarget, 8(35), 58443-58456 (2017-09-25)
Meningiomas are one of the most common tumors of the Central nervous system (CNS). This study aims to identify the autoantibody biomarkers in meningiomas using high-density human proteome arrays (~17,000 full-length recombinant human proteins). Screening of sera from 15 unaffected
Jin-Ying Lu et al.
Methods in molecular biology (Clifton, N.J.), 981, 151-165 (2013-02-06)
Emergence of proteome microarray provides a versatile platform to globally explore biological functions of broad significance. In the past decade, researchers have successfully fabricated functional proteome microarrays by printing individually purified proteins at a high-throughput, proteome-wide scale on one single
Shabarni Gupta et al.
Methods in molecular biology (Clifton, N.J.), 1619, 303-315 (2017-07-05)
Protein microarrays are platforms for studying protein-protein interactions and identifying disease-related self-antigens/autoantigens, which elicit an immune response in a high-throughput format. Protein arrays have been extensively used over the past two decades for several clinical applications. By using this platform

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