AB3212-I
Anti-Na+/HCO3- Cotransporter Antibody
from rabbit, purified by affinity chromatography
Synonyme(s) :
Electrogenic sodium bicarbonate cotransporter 1, Sodium bicarbonate cotransporter, Na(+)/HCO3(-) cotransporter, Solute carrier family 4 member 4, kNBC1
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Produit purifié par
affinity chromatography
Espèces réactives
rat, human
Réactivité de l'espèce (prédite par homologie)
rabbit (based on 100% sequence homology)
Technique(s)
immunohistochemistry: suitable
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... SLC4A4(8671)
Description générale
Electrogenic sodium bicarbonate cotransporter 1 (UniProt Q9Y6R1; also known as Sodium bicarbonate cotransporter, Na(+)/HCO3(-) co-transporter, Solute carrier family 4 member 4, kNBC1) is encoded by the SLC4A4 (also known as NBC, NBC1, NBCE1) gene (Gene ID 8671) in human. Bicarbonate (HCO3-) transporters are the principal regulators of pH in cells and play a vital role in acid-base movement in various tissues. There exist four types of HCO3- transporters, namely Cl-/-HCO3- exchangers, Na+/HCO3- co-transporters (NBCs), a K+/HCO3- co-transporter, and a Na+-driven Cl-/-HCO3- exchanger. SLC4A4 is a multi-pass membrane NBC with 10 transmembrane segments, 5 extracellular domains, and 6 intracellular domains, having both its N- and C-terminal (a.a. 1-468 and a.a. 987-1079, respectively) exposed intracellularly. NBCs mediate the coupled movement of sodium and bicarbonate ions, in a 3 to 1 stoichiometric ratio, across the plasma membrane. Multiple isoforms are reported as a result of alternative splicing, including NBCe1-A (kNBC1, kNBC-1; UniProt Q9JI66-2/Rat and Q9Y6R1-2/Human) that is mainly expressed in the kidney, NBCe1-B (bNBC1, pNBC-1; UniProt Q9JI66-1/Rat and Q9Y6R1-1/Human) expressed in the pancreas and other tissues, and NBCe1-C (b2NBC; UniProt Q9JI66-3/Rat and Q9Y6R1-5/Human) expressed in the brain.
Spécificité
Expected to react with all rat and human spliced isoforms reported by UniProt (Q9JI66 and Q9Y6R1, respectively).
Immunogène
Epitope: Amino acids 338-391
GST-tagged recombinant protein corresponding to amino acids 338-391 of human Na+/HCO3- cotransporter.
Application
Detect Na+/HCO3- Cotransporter using this Anti-Na+/HCO3- Cotransporter Antibody validated for use in Western Blotting, Immunohistochemistry.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Na+/HCO3- cotransporter in human cerebral cortex, kidney and pancreas tissues.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Ion Channels & Transporters
Qualité
Evaluated by Western Blotting in human kidney tissue lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Na+/HCO3- cotransporter in 10 µg of human kidney tissue lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Na+/HCO3- cotransporter in 10 µg of human kidney tissue lysate.
Description de la cible
~130 kDa observed. Uncharacterized band(s) may appear in some lysates.
Forme physique
Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Stockage et stabilité
Stable for 1 year at 2-8°C from date of receipt.
Autres remarques
Concentration: Please refer to lot specific datasheet.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Vous ne trouvez pas le bon produit ?
Essayez notre Outil de sélection de produits.
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Štefan Baňas et al.
International journal of molecular sciences, 25(7) (2024-04-13)
Kaempferol (KAE) is a natural flavonoid with powerful reactive oxygen species (ROS) scavenging properties and beneficial effects on ex vivo sperm functionality. In this paper, we studied the ability of KAE to prevent or ameliorate structural, functional or oxidative damage
Cary R Boyd-Shiwarski et al.
American journal of physiology. Renal physiology, 318(6), F1341-F1356 (2020-04-14)
We characterized mouse blood pressure and ion transport in the setting of commonly used rodent diets that drive K+ intake to the extremes of deficiency and excess. Male 129S2/Sv mice were fed either K+-deficient, control, high-K+ basic, or high-KCl diets
Filip Benko et al.
International journal of molecular sciences, 23(23) (2022-12-12)
Since the molecular similarities and differences among physiological capacitation and cryocapacitation have not been studied in detail, this study was designed to assess the gene and protein expression levels of the Cation channel of sperm (CatSper) 1 and 2, sodium
Štefan Baňas et al.
International journal of molecular sciences, 24(3) (2023-02-12)
Epicatechin (EPC) is a flavonoid belonging to the family of catechins; it has been described as a powerful scavenger of a wide spectrum of reactive oxygen species (ROS) and a modulator of ex vivo sperm vitality. In this study, we
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique