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Merck
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Key Documents

AB19012

Sigma-Aldrich

Anti-Laminin Antibody

Chemicon®, from rabbit

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

Forme d'anticorps

affinity purified immunoglobulin

Clone

polyclonal

Espèces réactives

human

Fabricant/nom de marque

Chemicon®

Technique(s)

ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Spécificité

This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against human serum proteins collagen and non-collagen extracellular matrix proteins to remove unwanted reactivities. Typically less than 1% cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. This antibody reacts with most mammalian laminins and has negligible cross-reactivity with Type I, II, III, IV, V or VI collagens or fibronectin. Non-specific cross reaction of anti-laminin antibodies with other human serum proteins or non-laminin extracellular matrix proteins is negligible.

Immunogène

Full length human laminin. Immunization occurred after single-band purity was assessed by SDS-PAGE.

Application

Detect Laminin using this Anti-Laminin Antibody validated for use in ELISA, IP, WB, IH.
Immunoblot: 1:500-1:2000

ELISA: 1:4000-1:8000

Immunohistochemistry: 1:50-1:200

Immunoprecipitation: Condition must be determined by the end user

Optimal working dilutions must be determined by the end user.

Forme physique

Affinity purified immunoglobulin. Liquid at 1 mg/mL in 0.125 M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA, pH 8.0 with 0.01% Sodium Azide.
Format: Purified

Stockage et stabilité

The undiluted antibody solution is stable for approximately 6 months stored between 2 and 8° C. For extended storage mix with an equal volume of glycerol, aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogrammes

Health hazard

Mention d'avertissement

Danger

Mentions de danger

Classification des risques

Repr. 1B

Code de la classe de stockage

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Dhruv Sareen et al.
PloS one, 4(10), e7630-e7630 (2009-11-10)
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Cathepsin-L influences the expression of extracellular matrix in lymphoid organs and plays a role in the regulation of thymic output and of peripheral T cell number.
Gabriela Lombardi, Dalia Burzyn, Juliana Mundi?ano, Paula Berguer, Pedro Bekinschtein et al.
Journal of immunology (Baltimore, Md. : 1950) (1950)
Selective matrix metalloproteinase inhibition with developing heart failure: effects on left ventricular function and structure.
Mary K King, Mytsi L Coker, Aaron Goldberg, James H McElmurray, Himali R Gunasinghe et al.
Circulation Research null
Extracellular matrix-mediated membrane-type 1 matrix metalloproteinase expression in pancreatic ductal cells is regulated by transforming growth factor-beta1.
Ottaviano, AJ; Sun, L; Ananthanarayanan, V; Munshi, HG
Cancer Research null
Notch3 null mutation in mice causes muscle hyperplasia by repetitive muscle regeneration.
Takeo Kitamoto,Kazunori Hanaoka
Stem Cells null

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