AB1726
Anti-Connexin 40 Antibody
Chemicon®, from rabbit
Synonyme(s) :
Cx40, Gap Junction alpha-5 Protein, CxA-5
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Produit purifié par
affinity chromatography
Espèces réactives
mouse, rat, human
Fabricant/nom de marque
Chemicon®
Technique(s)
immunohistochemistry: suitable
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... GJA5(2702)
Description générale
Mouse Connexin 40 is a 358 amino acid gap junction protein with a predicted M.W. of ~40 kDa. It is prominently expressed in lung, heart and skin (see reviews: Kumar & Giula 1996; White, 1995; Evans, 1994; Beyer , 1990; Henneman, 1992; Traub, 1994; Goliger & Paul 1994). One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. A connexon is composed of a hexamer of connexins.
Spécificité
Reactivity with other species has not been determined.
Reacts with mouse connexin 40.
Immunogène
Anti-Connexin 40 is made against a 19 amino acid peptide sequence within the C-terminal cytoplasmic domain of mouse C×40 (2). Peptide sequence is conserved in human and rat Cx40 (100%), chicken Cx42 (77%), human Cx50 (87%), rat Cx50 (81%) and sheep Cx49 (87%).
Application
Anti-Connexin 40 Antibody is an antibody against Cx40 for use in IHC & WB.
Immunohistochemistry Analysis: An 1:50 dilution of a representative lot detected Connexin 40 immunoreactivity associated with the gap junction between the cardiac myoepithial cells in mouse heart tissue sections.
Western Blot Analysis: An 1:1000 dilution of a representative lot detected Connexin 40 in human (HEK293, HeLa, HepG2, HUVEC, Jurkat) and murine (C2C12, HIN/3T3) cell lysates.
Western Blot Analysis: An 1:000 dilution of a representative lot detected Connexin 40 in human, mouse, and rat heart tissue homogenates, as well as in mouse brain tissue homogenates.
Optimal working dilutions must be determined by end user.
Western Blot Analysis: An 1:1000 dilution of a representative lot detected Connexin 40 in human (HEK293, HeLa, HepG2, HUVEC, Jurkat) and murine (C2C12, HIN/3T3) cell lysates.
Western Blot Analysis: An 1:000 dilution of a representative lot detected Connexin 40 in human, mouse, and rat heart tissue homogenates, as well as in mouse brain tissue homogenates.
Optimal working dilutions must be determined by end user.
Qualité
Evaluated by Western Blot on Jurkat lysates.
Western Blot Analysis: A 1:1000 dilution of this lot detected Connexin 40 in 10 μg of Jurkat lysates.
Western Blot Analysis: A 1:1000 dilution of this lot detected Connexin 40 in 10 μg of Jurkat lysates.
Description de la cible
40 kDa
Forme physique
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 15 mM NaCl containing 0.05% sodium azide.
Remarque sur l'analyse
Control
Placenta lysate.
Placenta lysate.
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Markus Boettcher et al.
Hypertension (Dallas, Tex. : 1979), 57(4), 802-808 (2011-03-02)
The endothelium-derived hyperpolarizing factor (EDHF) contributes critically to the regulation of vascular tone. Its dependency on direct signaling through myoendothelial gap junctions composed of connexins (Cx) is controversially discussed. We studied the impact of Cx40 in EDHF-type dilations in vivo
Volker J Schmidt et al.
PloS one, 8(11), e78782-e78782 (2013-11-16)
Gap junctions are involved in vascular growth and their expression pattern is modulated in response to hemodynamic conditions. They are clusters of intercellular channels formed by connexins (Cx) of which four subtypes are expressed in the cardiovascular system, namely Cx37
Connexin isoform expression in smooth muscle cells and endothelial cells of hamster cheek pouch arterioles and retractor feed arteries.
Hakim, CH; Jackson, WF; Segal, SS
Microcirculation (New York, N.Y. : 1994) (1994)
Florian Alonso et al.
Cardiovascular research, 87(1), 166-176 (2010-01-30)
Connexins (Cxs) play a role in the contractility of the aorta wall. We investigated how connexins of the endothelial cells (ECs; Cx37, Cx40) and smooth muscle cells (SMCs; Cx43, Cx45) of the aorta change during renin-dependent and -independent hypertension. We
Anja Hagen et al.
Cardiovascular research, 83(2), 405-415 (2009-01-30)
Ventricular arrhythmia is one of the most important causes of death in industrialized countries and often accompanies myocardial infarction and heart failure. In recent years modification of gap-junctional coupling has been proposed as a new antiarrhythmic principle. We wanted to
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