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Key Documents

AB1538

Sigma-Aldrich

Anti-Dopamine β Hydroxylase Antibody, NT

Chemicon®, from rabbit

Synonyme(s) :

DBH

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Produit purifié par

affinity chromatography

Espèces réactives

rat, human, bovine, sheep

Fabricant/nom de marque

Chemicon®

Technique(s)

immunohistochemistry: suitable
western blot: suitable

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... DBH(1621)

Spécificité

Dopamine Beta Hydroxylase (DbetaH) N-terminal. By immunoblot the antibody specifically reacts with a single (or double) band(s) of M.W. »70-75,000 in samples of SDS-solubilized, DTT-reduced human adrenal medulla subjected to SDS-PAGE.

Immunogène

Epitope: N-terminus
Synthetic peptide, CSAPRESPLPYHIPLDPEG-amide, cloned from human (DbetaH) cDNA (Nagatsu et al. 1990).

Application

Detect Dopamine β Hydroxylase using this Anti-Dopamine β Hydroxylase Antibody, N-terminus validated for use in IH & WB.
Immunoblotting: 1:250-1:1,000.

Immunohistochemistry: 1:250-1:1,000, staining was evaluated in the locus coeruleus of formaldehyde-fixed, cryopreserved human and monkey brain Showed weak staining on both human and monkey.

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors

Neuronal & Glial Markers

Forme physique

Liquid in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Stockage et stabilité

Maintain at -20 to -70°C in undiluted aliquots for up to six months. Avoid repeated freeze-thaw cycles.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1


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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

M E Da Vitoria Lobo et al.
Frontiers in pain research (Lausanne, Switzerland), 4, 1190440-1190440 (2023-06-16)
Chronic pain is a prevalent physically debilitating health-related morbidity. Frontline analgesics are inadequate, providing only partial pain relief in only a proportion of the patient cohort. Here, we explore whether alterations in spinal cord vascular perfusion are a factor in
Marie-Josée Wallman et al.
The European journal of neuroscience, 33(8), 1519-1532 (2011-03-08)
This study aimed to provide a first detailed description of the serotonin (5-hydroxytryptamine, 5-HT) innervation of the human basal ganglia under nonpathological conditions. We applied an immunohistochemical approach to postmortem human brain material with antibodies directed against the 5-HT transporter
Keita Harada et al.
Journal of neurochemistry, 114(2), 617-626 (2010-05-19)
Neurons and certain kinds of endocrine cells, such as adrenal medullary (AM) cells, have large dense-core vesicles (LDCVs) and synaptic vesicles or synaptic-like microvesicles (SLMVs). These secretory vesicles differ in Ca(2+) sensitivity and contain different signaling substances. We have recently
Georg Gussak et al.
JCI insight, 4(20) (2019-09-11)
Atrial fibrillation (AF) is the most common heart rhythm disorder and a major cause of stroke. Unfortunately, current therapies for AF are suboptimal, largely because the molecular mechanisms underlying AF are poorly understood. Since the autonomic nervous system is thought
Keita Harada et al.
Journal of neurochemistry, 158(2), 153-168 (2021-03-12)
γ-Aminobutyric acid (GABA) is thought to play a paracrine role in adrenal medullary chromaffin (AMC) cells. Comparative physiological and immunocytochemical approaches were used to address the issue of how the paracrine function of GABA in AMC cells is established. GABAA

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