499260

Osteopontin, His•Tag^®, Mouse, Recombinant, Mouse

• Synonyme(s) : rmOPN
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.45

Propriétés

• Pureté: ≥95% (SDS-PAGE)
• Niveau de qualité: 100
• Forme: lyophilized
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze
• Impuretés: ≤100 pg/μg Endotoxins (pg/μg rmOPN)
• Conditions d'expédition: ambient
• Température de stockage: −70°C

Description

Description générale

M.W. 65,000 and 30,000 under reducing conditions.

Recombinant, mouse osteopontin fused at the C-terminus to a His•Tag sequence and expressed in NSO-derived Leu¹⁷-Asn²⁹⁴ (Glu99Gly) murine myeloma cells. A secreted, highly acidic, Ca²⁺-binding, RGD-containing, phosphorylated glycoprotein. Chemotactic for macrophages, smooth muscle cells, endothelial cells, and glial cells. Has been shown to inhibit nitric oxide production and cytotoxicity by activated macrophages. Plays a significant role in inducing granulomas of various origin. OPN is also involved in the migration of embryonic fibroblasts, macrophages, and metastatic cells.

Recombinant, mouse osteopontin fused at the C-terminus to a His•Tag sequence and expressed in NSO-derived Leu¹⁷-Asn²⁹⁴ (Glu99Gly) murine myeloma cells. A secreted, highly acidic, calcium-binding, RGD-containing, phosphorylated glycoprotein. Osteopontin is expressed in several cell types including macrophages, fibroblasts, epithelial cells, T cells and osteoclasts in response to activation by cytokines, growth factors or inflammatory mediators. Mouse OPN cDNA encodes a 294 amino acid protein that has a 16 amino acid predicted signal peptide, which is cleaved to generate 278 amino acid mature protein. The mature protein contains an integrin binding arginine-glycine-aspartic acid (RGD) sequence. Exhibits some renoprotective actions in renal injury, such as increasing tolerance to acute ischemia, inhibiting inducible nitric oxide synthase, and reducing cell peroxide levels.

Actions biochimiques/physiologiques

~200 ng immobilized osteopontin/well will mediate the adhesion of ≥25% of the 293 cells plated at 10⁵ cells/well

Avertissement

Toxicity: Standard Handling (A)

Forme physique

Lyophilized from 0.2 µm sterile-filtered PBS, 50 µg BSA/µg rmOPN.

Reconstitution

Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 1 month at 4°C and for up to 3 months at -70°C. Avoid freeze/thaw cycles of stock solutions.

Reconstitute to ≥100 µg/ml with sterile PBS.

Autres remarques

Xie, Y., et al. 2001. Kidney Int.60, 1645.
Chiba, S., et al. 2000. Microbiol. Immunol. 44, 319.
Miyazaki, Y., et al. 1990. J. Biol. Chem. 265, 14432.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable