475911

Myeloperoxidase, Human Polymorphonuclear Leukocytes

A lysosomal enzyme that catalyzes oxidations by hydrogen peroxide, including MPO-chloride-mediated killing of microbes and tumor cells, inactivation of chemotactic factors, and cross-linking of proteins.

• Synonyme(s) : Myeloperoxidase, Human Polymorphonuclear Leukocytes, MPO
• Numéro CAS: 9003-99-0
• Numéro de classification (Commission des enzymes): 1.11.1.7 (BRENDA, IUBMB)
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.25

Propriétés

• Source biologique: human
• Niveau de qualité: 200
• Pureté: ≥95% (SDS-PAGE)
• Forme: lyophilized
• Activité spécifique: 150-200 units/mg protein
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze
• Solubilité: water: soluble (volume is lot-specific and can be found on the product label)
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• InChI: 1S/H2O3/c1-3-2/h1-2H
• Clé InChI: JSPLKZUTYZBBKA-UHFFFAOYSA-N

Description

Description générale

Native, human myeloperoxidase from polymorphonuclear leukocytes. A lysosomal enzyme that catalyzes oxidations by hydrogen peroxide, including MPO-chloride-mediated killing of microbes and tumor cells, inactivation of chemotactic factors, and cross-linking of proteins. MPO catalyzes the formation of hypochlorous acid that kills bacteria either by binding the halide ion to intracellular constituents or by oxidation of bacterial lipids and proteins.

Avertissement

Toxicity: Standard Handling (A)

Définition de l'unité

One unit is defined as the amount of enzyme that will reduce 1.0 µmol H₂O₂ per min at 25°C, pH 6.0.

Forme physique

Lyophilized from 100-700 mM NaCl, 50 mM sodium acetate buffer, pH 6.0.

Notes préparatoires

Further dilute in 100 mM NaCl, 50 mM sodium acetate, pH 6.0. Enzyme may precipitate out of solution in low ionic strength buffers or in distilled H₂O.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C or -70°C). Stock solutions are stable for up to 1 year at -20°C or -70°C.

Autres remarques

Nauseef, W.M., et al. 1998. J. Leukocyte Biol. 63, 264.
Panasenko, O.M., et al. 1994. Free Radic. Biol. Med. 16, 143.
Mulligan, M.S., et al. 1992. Br. J. Pharmacol.107, 1159.
Sharonov, B.P., and Churilova, I.V. 1992. Biochem. Biophys. Res. Commun.189, 1129.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1