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Merck
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Key Documents

374807

Sigma-Aldrich

Hemocyanin, Keyhole Limpet, Megathura crenulata, BES/Mg2+ Buffer

Hemocyanin, Keyhole Limpet, Megathura crenulata, BES/Mg2+ buffer, CAS 9013-72-3, is a large, multi-subunit, O2-carrying, metalloprotein that is used in the production of antibodies.

Synonyme(s) :

Hemocyanin, Keyhole Limpet, Megathura crenulata, BES/Mg2+ Buffer, KLH

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About This Item

Numéro CAS:
9013-72-3
Numéro MDL:
MFCD00131280
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.41

Niveau de qualité

100

Pureté

≥90% (size exclusion chromatography)

Forme

liquid

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

do not freeze

Couleur

clear blue-gray

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Description générale

Keyhole limpet hemocyanin (KLH) is a large, multi-subunit, O2-carrying, metalloprotein. It is isolated from a marine snail species called Megathura crenulate.

Actions biochimiques/physiologiques

Keyhole limpet hemocyanin (KLH) exhibits immunostimulatory properties and stimulates both cell-mediated and humoral responses in animals and humans. It serves as a vaccine adjuvant and facilitates protein conjugation. In addition, KLH acts as a carrier protein to produce antibodies as well as haptens such as peptides and oligosaccharides. It is also used in the treatment of bladder cancer.

Conditionnement

Please refer to vial label for lot-specific concentration.

Avertissement

Toxicity: Standard Handling (A)

Forme physique

In 10 mM MgSO₄, 5 mM BES buffer, 0.9% NaCl, pH 6.5. Sterile-filtered.

Notes préparatoires

KLH is an extremely large protein complex that has a tendency to aggregate and precipitate from solutions. Use a buffer when diluting KLH and maintain the magnesium concentration at ≥10 mM. Store KLH solutions at 4°C. DO NOT freeze, vortex or agitate to dissolve KLH. Freezing or agitating KLH will cause protein aggregation and precipitation.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Evaluation of human antibody responses to keyhole limpet hemocyanin on a carbohydrate microarray
Oyelaran O and Gildersleeve JC, et al.
4th International Conference on Biomedical Engineering in Vietnam, 285-294 (2010)
Oyindasola Oyelaran et al.
Proteomics. Clinical applications, 4(3), 285-294 (2010-12-08)
Keyhole limpet hemocyanin (KLH) is used as a vaccine adjuvant, as a carrier protein for small haptens, and as a treatment for bladder cancer. Immunization with KLH produces antibodies to tumor-associated carbohydrate antigens (TACAs) in animals, and these antibodies have
Erik H J G Aarntzen et al.
Cancer immunology, immunotherapy : CII, 61(11), 2003-2011 (2012-04-25)
Keyhole limpet hemocyanin (KLH) attracts biomedical interest because of its remarkable immunostimulatory properties. Currently, KLH is used as vaccine adjuvant, carrier protein for haptens and as local treatment for bladder cancer. Since a quantitative human anti-KLH assay is lacking, it

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