251520-M

DAF-FM DA

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM.

• Synonyme(s) : DAF-FM DA, 3-Amino, 4-aminomethyl-2ʹ,7ʹ-difluorofluorescein Diacetate
• Formule empirique (notation de Hill): C₂₅H₁₈F₂N₂O₇
• Numéro CAS: 254109-22-3
• Poids moléculaire : 496.42
• Numéro MDL: MFCD16872050
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.25

Propriétés

• Niveau de qualité: 100
• Pureté: ≥98% (HPLC)
• Forme: solution
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; protect from light
• Couleur: pale yellow
• Fluorescence: λ_ex ~500 nm; λ_em ~515 nm
• Température de stockage: −20°C
• InChI: 1S/C25H18F2N2O7/c1-10(30)33-20-8-18-13(6-15(20)26)25(12-4-5-17(29-3)23(28)22(12)24(32)36-25)14-7-16(27)21(34-11(2)31)9-19(14)35-18/h4-9,29H,28H2,1-3H3
• Clé InChI: BEVHTVRRVVEMEF-UHFFFAOYSA-N

Description

Description générale

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Intracellularly releases DAF-FM (Cat. No. 251515) by the action of esterases. Reacts with NO, in the presence of O₂, resulting in a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence is reported to be stable above pH 5.8.

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Releases DAF-FM (Cat. No. 251515) by the action of intracellular esterases. Reacts with NO, in the presence of oxygen, resulting in the formation of a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence intensity is reported to be stable above pH 5.8. Excitation max.: ~500 nm; emission max.: ~515 nm.

Actions biochimiques/physiologiques

Primary Target
Nitric oxide (NO) fluorescent indicator

Avertissement

Toxicity: Irritant (B)

Forme physique

A 5 mM solution in DMSO.

Reconstitution

Following initial thaw, aliquot and freeze at (-20°C). Use immediately after dilution; discard any unused portion.

Autres remarques

Kojima, H., et al. 2001. J. Neurochem.76, 1404.
Park. J.M., et al. 2001. Br. J. Pharmacol.132, 1876.
Itoh, Y., et al. 2000. Anal. Biochem.287, 203.
Kojima, H., et al. 1999. Angew Chem. Int. Ed.38, 3209.

Informations légales

Manufactured by Daiichi Pure Chemicals Co., Ltd

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): 188.6 °F - closed cup - (Dimethylsulfoxide)
• Point d'éclair (°C): 87 °C - closed cup - (Dimethylsulfoxide)