2384

Creatine Phosphokinase, Rabbit Skeletal Muscle

• Synonyme(s) : Creatine Phosphokinase, Rabbit Skeletal Muscle, rCPK
• Numéro CAS: 9001-15-4
• Numéro de classification (Commission des enzymes): 2.7.3.2 (BRENDA, IUBMB)
• Numéro MDL: MFCD00130876
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.21

Propriétés

• Source biologique: rabbit skeletal muscle
• Niveau de qualité: 200
• Forme: lyophilized
• Activité spécifique: ≥300 U/mg, 25 °C; 750 U/mg, 37 °C
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; desiccated (hygroscopic)
• Solubilité: 0.1 M imidazole buffer, pH 6.6: soluble; 0.5% NaHCO₃, pH 9.0: soluble
• Activité étrangère: ATPase, hexokinase, myokinase <0.001%
• Conditions d'expédition: ambient
• Température de stockage: −20°C

Description

Description générale

Native creatine phosphokinase from rabbit muscle. Catalyzes the conversion of creatine and ATP to creatine phosphate and ADP. Has an optimal pH of 9.0 for the forward reaction and 6.0-7.0 for the reverse reaction.

Native creatine phosphokinase from rabbit muscle. Catalyzes the reversible conversion of creatine and ATP to creatine phosphate and ADP. Has an optimal pH of 9.0 for the forward reaction and 6.0-7.0 for the reverse reaction.

Avertissement

Toxicity: Standard Handling (A)

Définition de l'unité

One unit is defined as the amount of enzyme that will catalyze the conversion of 1.0 µmol creatine phosphate per min at 25°C or 37°C, pH 6.8.

Reconstitution

Following reconstitution in 0.5% NaHCO₃, refrigerate (4°C). Do not freeze or shake, as SH groups may be oxidized and form linkages between protein molecules, resulting in precipitation, turbidity, and loss of activity. NaHCO₃ stock solutions are stable for 2-3 days at 4°C. Following reconstitution in 0.1 M imidazole buffer, aliquot and freeze (-20°C). Imidazole stock solutions are stable for up to 4 weeks at -20°C. Avoid freeze/thaw cycles, as 20-30% of activity will be lost with each freeze/thaw cycle.

Autres remarques

Bessman, S.P. 1987. Anal. Biochem.161, 519.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable