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217594

Sigma-Aldrich

Anti-CD44 Rat mAb (A020)

liquid, clone A020, Calbiochem®

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rat

Niveau de qualité

100

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

A020, monoclonal

Forme

liquid

Contient

≤0.1% sodium azide as preservative

Espèces réactives

canine, bovine, human, rabbit, porcine, mouse

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

OK to freeze
avoid repeated freeze/thaw cycles

Isotype

IgG2b

Conditions d'expédition

wet ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

bovine ... Cd44(281057)
dog ... Cd44(403939)
human ... CD44(960)
mouse ... Cd44(12505)
pig ... Cd44(100525150)

Description générale

Anti-CD44, rat monoclonal, clone A020, recognizes the ~85-95 kDa CD44 protein (gp90) in human and GP85 in mouse. It is validated for ELISA, IF, IP, RIA, WB, and IHC.
Rat monoclonal antibody purified by ammonium sulfate precipitation and DEAE chromatography. Recognizes the ~85-95 kDa CD44 protein.
Recognizes the ~85-95 kDa CD44 protein (gp90) in human and GP85 or Pgp-1 in mouse.

Immunogène

Human
purified human lymphocyte CD44

Application

ELISA (1:2000)

Flow Cytometry (1:1000)

Immunoblotting (1:1000)

Immunofluorescence (1:200)

Immunohistochemistry (1:250)

Immunoprecipitation (1:200)

Neutralization Studies (see comments and application references)

Radioimmunoassay (1:5000)

Conditionnement

Please refer to vial label for lot-specific concentration.

Avertissement

Toxicity: Standard Handling (A)

Forme physique

In 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Autres remarques

Iida, N., and Bourguignon, L.Y.W. 1995. J. Cell Physiol.162, 121.
Bourguignon, L.Y.W., et al. 1993. J. Immunol.151, 6634.
The antibody specifically recognizes and reacts with a common determinant of the CD44 class of glycoproteins. This antibody has been reported to block the interaction between hyaluronan and CD44 (see application references). Variables associated with assay conditions will dictate the proper working dilution.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Eun-Ju Chang et al.
Journal of cell science, 120(Pt 1), 166-176 (2006-12-14)
The differentiation of osteoclasts, cells specialized for bone resorption, is governed by two key factors, macrophage colony stimulating factor (M-CSF) and receptor activator of nuclear factor kappaB ligand (RANKL). The extracellular matrix (ECM) is an important factor influencing cell fate.
Daniel S Puperi et al.
Biomacromolecules, 17(5), 1766-1775 (2016-04-28)
Advanced tissue engineered heart valves must be constructed from multiple materials to better mimic the heterogeneity found in the native valve. The trilayered structure of aortic valves provides the ability to open and close consistently over a full human lifetime
Jay J Cao et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 20(1), 30-40 (2004-12-28)
HA activates CD44 to stimulate RANKL expression in bone marrow stromal cells. HA stimulation of RANKL is blocked by anti-CD44 antibody and is absent in cells from CD44(-/-) mice. CD44(-/-) mice exhibit thicker cortical bone and a smaller medullary cavity
Shibnath Ghatak et al.
The Journal of biological chemistry, 280(10), 8875-8883 (2005-01-06)
Hyaluronan is enriched in many types of human cancers, and manipulations of hyaluronan expression or interactions have a major influence on tumor progression in animal models. Increased ErbB2 activity is characteristic of several cancers and is responsible for many aspects
Mengya Zhao et al.
Cell reports, 42(9), 113038-113038 (2023-08-25)
Chronic neurodegeneration and acute injuries lead to neuron losses via diverse processes. We compared retinal ganglion cell (RGC) responses between chronic glaucomatous conditions and the acute injury model. Among major RGC subclasses, αRGCs and intrinsically photosensitive RGCs (ipRGCs) preferentially survive
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