189737

Streptavidin, Phycoerythrin Conjugate

This Streptavidin, Phycoerythrin Conjugate is validated for use in Enzyme Immunoassay, Flow Cytometry.

• Code UNSPSC : 41116133
• Nomenclature NACRES : NA.41

Propriétés

• Niveau de qualité: 100
• Forme: liquid
• Contient: ≤0.1% sodium azide as preservative
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: do not freeze; protect from light
• Fluorescence: λ_ex 500 nm; λ_em 575 nm
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C

Description

Description générale

Streptavidin conjugated to R-phycoerythrin. Specific for biotin, biotinylated proteins, or biotinylated antibodies.

Application

Enzyme Immunoassay (see comments)

Flow Cytometry (1-3 µg per 1 x 10⁶ cells in 100 µl)

Conditionnement

Please refer to vial label for lot-specific concentration.

Avertissement

Toxicity: Standard Handling (A)

Forme physique

In 150 mM NaCl, 50 mM sodium phosphate, 1% BSA, 0.1% glycerol, pH 7.6. Ammonium sulfate free.

Reconstitution

Do not dilute the entire vial at one time. Dilute only as needed in 50 mM sodium phosphate buffer, pH 7.2, 2-5 mg/ml of BSA or other physiological buffer.

Autres remarques

Lindmo, T., et al. 1990. J. Immunol. Methods126, 183.
Starling, J.J., et al. 1988. Cancer Res.48, 6211.

The phycobiliprotein used in making this conjugate had a M.W. of 240,000. Molar extinction coefficient at 565 nm: 1.9 x 10⁶. Excitation Max: 500 nm emission max: 575 nm.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable