16-282
Anti-Phosphotyrosine Antibody, clone 4G10®, Magnetic Bead Conjugate
clone 4G10®, from mouse
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified antibody
Type de produit anticorps
primary antibodies
Clone
4G10®, monoclonal
Réactivité de l'espèce (prédite par homologie)
all
Technique(s)
immunoprecipitation (IP): suitable
Isotype
IgG2bκ
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
phosphorylation (pTyr)
Informations sur le gène
human ... PID1(55022)
Description générale
Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine.).The advent of anti-phosphotyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.
Spécificité
This antibody recognizes tyrosine-phosphorylated proteins from all species
Immunogène
Phosphotyramine-KLH
Application
Detect Phosphotyrosine using this Anti-Phosphotyrosine Antibody, clone 4G10, Magnetic Bead Conjugate validated for use in IP.
Research Category
Signaling
Signaling
Research Sub Category
General Post-translation Modification
General Post-translation Modification
Qualité
100 μg of NIH3T3 whole cell lysate stimulated with 100 ng/mL PDGF (10 minutes) was immunoprecipitated (IP) using either anti-mouse IgG (Cat. No. 12-371) (lane 1), or Anti-Phosphotyrosine, Magnetic Bead Conjugate
(Lane 2).
(Lane 2).
Description de la cible
Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
Forme physique
20% slurry; 1xPBS, 0.05% sodium azide. Sufficient for 10 IPs.
Format: Purified
Protein G-Sepharose Chromatography
Stockage et stabilité
Stable for 1 year at 2-8ºC from date of receipt.
Note: DO NOT FREEZE
Note: DO NOT FREEZE
Remarque sur l'analyse
Control
NIH3T3 whole cell lysate stimulated with 50 ng/mL PDGF (5 minutes)
NIH3T3 whole cell lysate stimulated with 50 ng/mL PDGF (5 minutes)
Informations légales
4G10 is a registered trademark of Upstate Group, Inc.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Certificats d'analyse (COA)
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
M Whitfield et al.
Reproduction (Cambridge, England), 154(6), 827-842 (2017-10-04)
Lipid metabolism disorders (dyslipidemia) are causes of male infertility, but little is known about their impact on male gametes when considering post-testicular maturation events, given that studies concentrate most often on endocrine dysfunctions and testicular consequences. In this study, three-month-old
F Saez et al.
Andrology, 7(5), 654-661 (2019-06-05)
Lipid metabolic disorders (dyslipidemia) are constantly increasing in occidental societies and lead to the development of pathologies such as obesity, diabetes, and metabolic syndrome. It has been demonstrated that dyslipidemia can alter the reproductive function. Animal models have recently been
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