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Key Documents

1.52027

Supelco

Chromolith® NH2 (monolithic) HPLC Columns

L × I.D. 50 mm x 4.6 mm HPLC Column

Synonyme(s) :

Chromolith® SpeedROD NH2 50-4.6 HPLC column

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About This Item

Code UNSPSC :
41115700
Nomenclature NACRES :
SB.52

product name

Chromolith® NH2, L × I.D. 50 mm x 4.6 mm HPLC column

Matériaux

PEEK column

Niveau de qualité

Agence

suitable for USP L8

Gamme de produits

Chromolith®

Caractéristiques

endcapped: no

Conditionnement

1 ea of

Ampleur du marquage

5% Carbon loading

Paramètres

200 bar pressure
45 °C max. temp.

Technique(s)

HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (Low - Medium)

Colonne, L × D.I.

5 cm × 4.6 mm

Matrice

monolithic silica

Groupe de la matrice active

NH2 bonding phase

Dimension de pores

13 nm mesoporosity
2 μm macroporosity

operating pH range

2-7.5

Technique de séparation

hydrophilic interaction (HILIC)
normal phase
reversed phase

Température de stockage

no temp limit

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Description générale

Chromolith® columns are made of monolithic silica with a characteristic bimodal pore structure.

Chromolith® NH2 columns have macropores with 2 μm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 13 nm (130 Å). Chromolith® NH2 columns fall under the USP L8 Classification. The column efficiency is comparable to a 5 μm particle size fully porous particulate column. Chromolith® NH2 columns are suitable for LC-MS applications.
The column dimension of 50 mm length and 4.6 mm I.D. is the best choice for fast separations on any HPLC System.

The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity

The recommended Guard-columns are:
Chromolith® NH2 5-4.6 guard cartridges (3 pieces) [1.52030] with the Chromolith® Guard Cartridge Holder 5 mm x 4.6 mm [1.52032]
The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]

Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns

All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Remarque sur l'analyse

Pressure: ≤ 10 bar
Performance (2-nitroanisol)
Tailing (USP): 0.85 - 1.30 %
Theoretical Plates (N/m) (Cytosine): ≥ 80000
Capacity factor (Cytosine): ≥ 1.1

Informations légales

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificats d'analyse (COA)

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Articles

Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.

Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.

Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.

Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.

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