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1.00497

Sigma-Aldrich

AFB-Color modified

Staining kit for the detection of acid-fast bacteria (AFB) by hot staining method

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About This Item

Code UNSPSC :
41116130
Nomenclature NACRES :
NA.74

Niveau de qualité

400

IVD

for in vitro diagnostic use

Technique(s)

microbe id | staining: suitable

Application(s)

clinical testing
diagnostic assay manufacturing
hematology
histology

Température de stockage

15-25°C

Adéquation

(Acid-fast bacteria)

Description générale

The AFB-Color modified - Staining kit for the detection of acid-fast bacteria (AFB) by hot staining method, is used for human-medical cell diagnosis and serves the purpose of the bacteriological investigation of sample material of human origin, for example smears of enriched bacterial cultures.
AFB-Color modified staining set uses the classical Ziehl-Neelsen hot staining procedure and can be used for staining on the staining rack. The cell wall of acid-fast bacteria has a high proportion of wax and lipids and hence absorbs dyes only very slowly. The most efficient staining method is the hot staining according to Ziehl-Neelsen. In this method, carbolfuchsin solution is applied to the specimen and heated. This heating process accelerates the rate at which the fuchsin dye is absorbed and thus the formation of the mycolate-fuchsin complex in the cell wall. Once the acid-fast bacteria have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol. Accordingly, acid-fast bacteria are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye. This AFB-Color modified staining kit uses methylene blue as counterstain.
Pretreatment of the specimens with Sputofluol™ dissolves the bacteria from the surrounding viscid sputum and cell material.
The package is sufficient for approx. 250 applications. This product is registered as IVD and CE marked.For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Application

Used to detect acid-fast bacteria" (AFB hot staining)

Remarque sur l'analyse

Suitability for microscopypasses test acid-fast bacteria red Background blue

Informations légales

SPUTOFLUOL is a trademark of Merck KGaA, Darmstadt, Germany

Mention d'avertissement

Danger

Classification des risques

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 2 - Met. Corr. 1 - Muta. 2 - Skin Corr. 1B

Code de la classe de stockage

3 - Flammable liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

69.8 °F

Point d'éclair (°C)

21 °C

Certificats d'analyse (COA)

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