07-215
Anti-dimethyl-Histone H3 (Arg26) Antibody
from rabbit, purified by affinity chromatography
Synonyme(s) :
H3R26me2, Histone H3 (di methyl R26), H3 histone family, member T, histone 3, H3, histone cluster 3, H3
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Produit purifié par
affinity chromatography
Espèces réactives
vertebrates, Tetrahymena sp., Xenopus, human
Technique(s)
multiplexing: suitable
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
dimethylation (Arg26)
Informations sur le gène
human ... H3C1(8350)
Description générale
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Spécificité
Recognizes CARM1 in vitro methylated recombinant Histone H3. The immunizing sequence is conserved from Tetrahymena through human therefore a broad cross reactivity is expected.
Recognizes dimethylated Histone H3 at and around arginine 26, MW ~17 kDa. Possible slight reactivity with monomethyl histone H3 (Arg26).
Immunogène
Epitope: Dimethylated Histone H3 (Arg26)
KLH-conjugated synthetic peptide (ATKAA(di-Me)RKSAPA-C) corresponding to amino acids 21-31 of Human Histone H3.
Application
Anti-dimethyl-Histone H3 (Arg26) Antibody is a rabbit polyclonal antibody for detection of dimethyl-Histone H3 (Arg26) also known as H3R26me2, Histone H3 (di methyl R26) & has been validated in WB, Mplex.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
SNAP I.D. (Western Blotting Technique):
A previous lot of this antibody worked successfully using the SNAP I.D. western blotting device and technique.
A previous lot of this antibody worked successfully using the SNAP I.D. western blotting device and technique.
Qualité
Evaluated by western blot on untreated and CARM1 in vitro methylated recombinant Histone H3.
Western Blot Analysis: 1:8000 dilution of this antibody detected dimethyl-Histone H3 (Arg26) in untreated and CARM1 in vitro methylated recombinant Histone H3.
Western Blot Analysis: 1:8000 dilution of this antibody detected dimethyl-Histone H3 (Arg26) in untreated and CARM1 in vitro methylated recombinant Histone H3.
Description de la cible
17kDa
Forme physique
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl, with 0.05% sodium azide.
Stockage et stabilité
Stable for 1 year at 2-8ºC from date of receipt.
Remarque sur l'analyse
Control
CARM1 in vitro methylated recombinant Histone H3.
CARM1 in vitro methylated recombinant Histone H3.
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Monica Mann et al.
Carcinogenesis, 34(7), 1468-1475 (2013-03-15)
Estrogen receptor alpha (ERα) is implicated in the initiation and progression of breast cancer and its transcription depends on the modulation of epigenetic changes at target gene promoters via coregulators. There is a critical need to understand the molecular mechanism(s)
Adam Burton et al.
Cell reports, 5(3), 687-701 (2013-11-05)
Cell plasticity or potency is necessary for the formation of multiple cell types. The mechanisms underlying this plasticity are largely unknown. Preimplantation mouse embryos undergo drastic changes in cellular potency, starting with the totipotent zygote through to the formation of
Lifang Niu et al.
Plant physiology, 148(1), 490-503 (2008-07-29)
CARM1/PRMT4 (for COACTIVATOR-ASSOCIATED ARGININE METHYLTRANSFERASE1/PROTEIN ARGININE METHYLTRANSFERASE4) catalyzes asymmetric dimethylation on arginine (Arg), and its functions in gene regulation is understood only in animal systems. Here, we describe AtPRMT4a and AtPRMT4b as a pair of Arabidopsis (Arabidopsis thaliana) homologs of
B Ruthrotha Selvi et al.
The Journal of biological chemistry, 285(10), 7143-7152 (2009-12-22)
Methylation of the arginine residues of histones by methyltransferases has important consequences for chromatin structure and gene regulation; however, the molecular mechanism(s) of methyltransferase regulation is still unclear, as is the biological significance of methylation at particular arginine residues. Here
Coactivator-associated arginine methyltransferase 1 (CARM1) is a positive regulator of the Cyclin E1 gene.
El Messaoudi, S; Fabbrizio, E; Rodriguez, C; Chuchana, P; Fauquier, L; Cheng, D; Theillet et al.
Proceedings of the National Academy of Sciences of the USA null
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