07-1404
Anti-GLUT-4 Antibody, C-terminus
from rabbit, purified by affinity chromatography
Synonyme(s) :
Solute carrier family 2, facilitated glucose transporter member 4, Glucose transporter type 4, insulin-responsive, GLUT-4
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Produit purifié par
affinity chromatography
Espèces réactives
mouse, human
Réactivité de l'espèce (prédite par homologie)
rat (based on 100% sequence homology)
Technique(s)
immunohistochemistry: suitable
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... SLC2A4(6517)
Description générale
The protein called GLUT-4, or more correctly Solute carrier family 2, facilitated glucose transporter member 4 and encoded by the human gene SLC2A4/GLUT4 is a primary insulin regulated facilitative glucose transporter found primarily in skeletal and cardiac muscle, as well as brown and white fat but is also expressed in other tissues such as brain. In the brain GLUT4 is found exclusively in neurons. GLUT-4 is facilitative because it is not always present on the cell plasma membrane. GLUT-4 only localizes to the plasma membrane upon insulin induction. GLUT-4 is localized to the perinuclear region in resting cells. Mutations in GLUT-4 are a cause of one form of non-insulin dependent diabetes mellitus. GLUT4 function as well as its transcriptional regulation is highly conserved throughout the vertebrate species.
Spécificité
Glucose transporter 4 (GLUT-4) is a protein present in rat skeletal muscle, heart and adipose tissues. GLUT-4 is highly conserved between species, and the peptide sequence selected for production of this antibody is identical in rat, human (amino acids 498-510), and mouse. This antibody shows no cross-reactivity of HepG2 type transporter from rat brain or other glucose transporters.
Immunogène
Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminus of human GLUT-4.
Application
Detect GLUT-4 using this Anti-GLUT-4 Antibody, C-terminus validated for use in WB, IH(P), ELISA.
Immunohistochemistry Analysis: A 1:50-2,000 dilution from a representative lot detected GLUT-4 in human cardiac myocytes and human skeletal muscle myocytes.
Research Category
Signaling
Signaling
Research Sub Category
Insulin/Energy Signaling
Insulin/Energy Signaling
Qualité
Evaluated by Western Blotting in NIH3T3 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GLUT-4 in 10 µg of NIH3T3 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GLUT-4 in 10 µg of NIH3T3 cell lysate.
Description de la cible
~58 kDa observed
Liaison
Replaces: AB1346
Forme physique
Affinity purified
Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.
Stockage et stabilité
Stable for 1 year at 2-8°C from date of receipt.
Remarque sur l'analyse
Control
NIH3T3 cell lysate
NIH3T3 cell lysate
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Réf. du produit
Description
Tarif
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 2
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Dolly Al Koborssy et al.
Frontiers in neuroscience, 8, 333-333 (2014-11-18)
In the brain, glucose homeostasis of extracellular fluid is crucial to the point that systems specifically dedicated to glucose sensing are found in areas involved in energy regulation and feeding behavior. Olfaction is a major sensory modality regulating food consumption.
Doxorubicin Induces Inflammatory Modulation and Metabolic Dysregulation in Diabetic Skeletal Muscle.
Rashmi Supriya et al.
Frontiers in physiology, 7, 323-323 (2016-08-12)
Anti-cancer agent doxorubicin (DOX) has been demonstrated to worsen insulin signaling, engender muscle atrophy, trigger pro-inflammation, and induce a shift to anaerobic glycolytic metabolism in skeletal muscle. The myotoxicity of DOX in diabetic skeletal muscle remains largely unclear. This study
Insulin and insulin-like growth factor I up-regulate GLUT4 gene expression in fetal brown adipocytes, in a phosphoinositide 3-kinase-dependent manner.
Valverde, A M, et al.
The Biochemical Journal, 337 ( Pt 3), 397-405 (1999)
ChREBP, a glucose-responsive transcriptional factor, enhances glucose metabolism to support biosynthesis in human cytomegalovirus-infected cells.
Yu, Y; Maguire, TG; Alwine, JC
Proceedings of the National Academy of Sciences of the USA null
Ceramide mediates insulin resistance by tumor necrosis factor-alpha in brown adipocytes by maintaining Akt in an inactive dephosphorylated state.
Teruel, T, et al.
Diabetes, 50, 2563-2571 (2001)
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