06-991
Anti-PKCε Antibody
Upstate®, from rabbit
Synonyme(s) :
protein kinase C, epsilon
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
polyclonal
Espèces réactives
rat, human, mouse
Fabricant/nom de marque
Upstate®
Technique(s)
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Isotype
IgG
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... PRKCE(5581)
Description générale
Members of the protein kinase C family fall into three categories: conventional (PKC alpha, beta I, beta II, gamma), novel (delta, epsilon, eta, mu, theta), and atypical (lambda, zeta). Conventional PKC isoforms are dependent on calcium and diacylglycerol (DAG) for their activity, whereas novel PKC isoforms are calcium dependent, and atypical PKC isoforms are calcium and DAG independent.
Spécificité
Predicted to cross-react with rabbit.
Recognizes PKCε, Mr 95 kDa.
Immunogène
KLH-conjugated, synthetic peptide corresponding to the C-terminal variable (V5) region, amino acids 726-737 (KGFSYFGEDLMP), of mouse Protein Kinase C Epsilon (PKCε). The immunizing sequence is identical in human, mouse, rat and rabbit.
Application
Detect PKCε using this Anti-PKCε Antibody validated for use in IH, IP & WB.
Immunoprecipitation:
An independent lab has reported this antibody immunoprecipitates PKCε.
Immunohistochemistry:
An independent lab has reported this antibody detects PKCε.
An independent lab has reported this antibody immunoprecipitates PKCε.
Immunohistochemistry:
An independent lab has reported this antibody detects PKCε.
Research Category
Signaling
Signaling
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
Qualité
Routinely evaluated by Western Blot on Jurkat cell lysate.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected PKCε in Jurkat cell lysate. 0.5-2 μg/mL of a previous lot detected PKCε in a RIPA lysate from Jurkat cells, mouse brain extract and rat brain extract.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected PKCε in Jurkat cell lysate. 0.5-2 μg/mL of a previous lot detected PKCε in a RIPA lysate from Jurkat cells, mouse brain extract and rat brain extract.
Description de la cible
Approx. 95
Forme physique
Format: Purified
Protein A purified
Purified rabbit IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide, and 30% glycerol.
Store at -20°C.
Store at -20°C.
Stockage et stabilité
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Remarque sur l'analyse
Control
Positive Antigen Control: Catalog #12-303, Jurkat cell lysate.
Positive Antigen Control: Catalog #12-303, Jurkat cell lysate.
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informations légales
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Protein kinase C epsilon activates lens mitochondrial cytochrome c oxidase subunit IV during hypoxia.
Michael Barnett, Dingbo Lin, Vladimir Akoyev, Lloyd Willard, Dolores Takemoto et al.
Experimental Eye Research null
Post-ischaemic activation of kinases in the pre-conditioning-like cardioprotective effect of the platelet-activating factor.
Penna C, Mognetti B, Tullio F, Gattullo D, Mancardi D, Moro F, Pagliaro P, Alloatti G
Acta Physiologica (Oxford, England) null
Platelet-activating factor induces cardioprotection in isolated rat heart akin to ischemic preconditioning: role of phosphoinositide 3-kinase and protein kinase C activation.
Penna, C; Alloatti, G; Cappello, S; Gattullo, D; Berta, G; Mognetti, B; Losano, G; Pagliaro, P
American Journal of Physiology. Heart and Circulatory Physiology null
Katherine Holmes et al.
PloS one, 5(7), e11435-e11435 (2010-07-14)
Vascular endothelial growth factor (VEGF) has previously been shown to upregulate the expression of the endogenous calcineurin inhibitor, regulator of calcineurin 1, variant 4 (RCAN1.4). The aim of this study was to determine the role and regulation of VEGF-mediated RCAN1.4
Two conventional protein kinase C isoforms, alpha and beta I, are involved in the ATP-induced activation of volume-regulated anion channel and glutamate release in cultured astrocytes.
Alena Rudkouskaya,Artur Chernoguz,Renee E Haskew-Layton,Alexander A Mongin
Journal of Neurochemistry null
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