06-418
Anti-NFκB p65 Antibody, CT
Upstate®, from rabbit
Synonyme(s) :
Nuclear factor NF-kappa-B p65 subunit, v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear
factor of kappa light polypeptide gene enhancer in B-cells 3 (p65)), v-rel reticuloendotheliosis viral oncogene homolog A (avian), v-rel r
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
purified antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Espèces réactives
mouse, rat, human
Fabricant/nom de marque
Upstate®
Technique(s)
electrophoretic mobility shift assay: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Isotype
IgG
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
dry ice
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... NFKB1(4790)
Description générale
The transcription factor NFκB (Nuclear Factor κB) is involved in the expression and regulation of a number of important cellular and physiological processes such as growth, development, apoptosis, immune and inflammatory response, and activation of various viral promoters including human immunodeficiency virus long terminal repeats. NFκB represents a group of structurally related and evolutionarily conserved proteins related to the proto-oncogene c-Rel with five members in mammals that include Rel (cRel), RelA (p65), RelB, NFκB1 (p50 and its precursor p105), and NFκB2 (p52 and its precursor p100). NFκB/Rel proteins exist as homo- or heterodimers to form transcriptionally competent or repressive complexes. Although most NFκB dimers are activators of transcription, the p50/50 and p52/52 homodimers can repress the transcription of their target genes. The p50/p65 heterodimer of NFκB is the most abundant in cells.
Spécificité
NFκB p65, Mr 65 kDa
Immunogène
Epitope: C-terminus
KLH-conjugated, synthetic peptide corresponding to the 13 C-terminal amino acids (C-DMDFSALLSQISS) of the p65 subunit of human NFκB containing an N-terminal cysteine for conjugation. The immunizing sequence is identical in mouse.
Application
Anti-NFκB p65 Antibody, CT is a Rabbit Polyclonal Antibody for detection of NFκB p65 also known as Nuclear factor NF-kappa-B p65 subunit & has been validated in EMSA, IP & WB.
Immunoprecipitation:
4 µg of a previous lot immunoprecipitated NFκB p65 from 500 μg of a human A431 carcinoma cell lysate.
4 µg of a previous lot immunoprecipitated NFκB p65 from 500 μg of a human A431 carcinoma cell lysate.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Qualité
Routinely evaluated by Western Blot on RIPA lysates from A431 cells.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected NFkB p65 in RIPA lysates from A431 cells.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected NFkB p65 in RIPA lysates from A431 cells.
Description de la cible
65 kDa
Liaison
Replaces: 04-1008; 04-235
Forme physique
Format: Purified
Protein A chromatography
Purified rabbit polyclonal IgG in 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol. Liquid at -20°C.
Stockage et stabilité
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
Remarque sur l'analyse
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informations légales
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Description
Tarif
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
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Csaba F László et al.
Photochemistry and photobiology, 84(6), 1564-1568 (2008-07-17)
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Yu-Chen Hou et al.
American journal of physiology. Lung cellular and molecular physiology, 302(1), L174-L183 (2011-10-18)
Lung epithelial cells are important barriers in the respiratory system that provoke inflammatory responses through nuclear factor (NF)-κB activation to prevent pathogens from invading the body. Lipopolysaccharide (LPS) is a common pathogen-associated stimulus that activates IκB kinase (IKK) to regulate
Hongying Wang et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 24(6), 1971-1980 (2010-01-13)
Proteinase-activated receptors (PARs) are involved in both inflammation and tumorigenesis in epithelial cells. Interleukin (IL)-8 is a potent chemoattractant and is also involved in angiogenesis. The molecular mechanism whereby PARs induce epithelial IL-8 expression is not known. In HT-29 colonic
Wai Chi Ho et al.
Cancer research, 65(10), 4273-4281 (2005-05-19)
The primary goal of chemotherapy is to cause cancer cell death. However, a side effect of many commonly used chemotherapeutic drugs is the activation of nuclear factor-kappaB (NF-kappaB), a potent inducer of antiapoptotic genes, which may blunt the therapeutic efficacy
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