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Merck
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Key Documents

05-594

Sigma-Aldrich

Anti-HSP90 Antibody

clone D7a, Upstate®, from mouse

Synonyme(s) :

Anti-EL52, Anti-HEL-S-65p, Anti-HSP86, Anti-HSP89A, Anti-HSP90A, Anti-HSP90N, Anti-HSPC1, Anti-HSPCA, Anti-HSPCAL1, Anti-HSPCAL4, Anti-HSPN, Anti-Hsp103, Anti-Hsp89, Anti-Hsp90, Anti-LAP-2, Anti-LAP2

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

D7a, monoclonal

Espèces réactives

human, rabbit, pig, mouse, rat, bovine, chicken

Conditionnement

antibody small pack of 25 μg

Fabricant/nom de marque

Upstate®

Technique(s)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotype

IgG

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

ambient

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... HSP90AA1(3320)

Spécificité

Recognizes HSP90.

Immunogène

Full-length protein purified from chicken brain; clone D7a

Application

This Anti-HSP90 Antibody is validated for use in IP, WB for the detection of HSP90.

Qualité

routinely evaluated by immunoblot on RIPA lysates from mouse 3T3/A31 fibroblasts or human A431 cells

Description de la cible

90 kDa

Forme physique

Format: Purified
Protein G Purified immunoglobulin in 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4.

Remarque sur l'analyse

Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Autres remarques

Please refer to lot specific datasheet.

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Jason M Shapiro et al.
PloS one, 11(11), e0165227-e0165227 (2016-11-02)
We previously isolated AF20, a murine monoclonal antibody that recognizes a cell surface glycoprotein of approximately 90-110 kDa. The AF20 antigen is specifically expressed in human hepatoma and colon cancer cell lines, and thus could serve as a cancer biomarker.
The hsp90-based chaperone system: involvement in signal transduction from a variety of hormone and growth factor receptors.
Pratt, W B
Proceedings of the Society For Experimental Biology and Medicine. Society For Experimental Biology and Medicine, 217, 420-434 (1998)
Miquéias Lopes-Pacheco et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 41(6), 2194-2210 (2017-04-28)
Premature degradation of mutated cystic fibrosis transmembrane conductance regulator (CFTR) protein causes cystic fibrosis (CF), the commonest Mendelian disease in Caucasians. Despite recent advances in precision medicines for CF patients, many CFTR mutants have not been characterized and the effects
Interaction between the Rous sarcoma virus transforming protein and two cellular phosphoproteins: analysis of the turnover and distribution of this complex.
Brugge, J, et al.
Molecular and cellular biology, 3, 9-19 (1983)
Qingming Kong et al.
Proteome science, 8, 29-29 (2010-06-11)
Infectious bronchitis virus (IBV) is the coronavirus of domestic chickens causing major economic losses to the poultry industry. Because of the complexity of the IBV life cycle and the small number of viral structural proteins, important virus-host relationships likely remain

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