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Key Documents

05-584

Sigma-Aldrich

Anti-Tie2/TEK Antibody, clone Ab33

clone Ab33, Upstate®, from mouse

Synonyme(s) :

CD202b antigen, TEK tyrosine kinase, endothelial, Tunica interna endothelial cell kinase, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE-2, p140 TEK, soluble TIE2 variant 1, soluble TIE2 variant 2, venous malformations, multip

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

100

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

Ab33, monoclonal

Espèces réactives

human, rat, pig, mouse

Fabricant/nom de marque

Upstate®

Technique(s)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotype

IgG1κ

Numéro d'accès NCBI

NM_000459

Numéro d'accès UniProt

Q02763

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... TEK(7010)

Description générale

TIE2 (tyrosine kinase with Ig and EGF homology domains 2) is expressed almost exclusively in endothelial cells in mice, rats and humans. This receptor possesses a unique extracellular domain containing two immunoglobulin-like loops separated by three epidermal growth factor-like repeats that are connected to three fibronectin type III-like repeats. The ligand for the receptor is Angiopoietin 1. Defects in TIE2 are associated with inherited venous malformations; the TIE2 signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis.

Spécificité

Recognizes Tie2/TEK, Mr 145kDa

Immunogène

Fusion protein corresponding to residues 1-745 of human Tie2/TEK with a C-terminal His6-tag purified from infected SF-9 cells. Clone Ab33

Application

Immunoprecipitation: This antibody immunoprecipitated Tie2/TEK, as reported by an independent laboratory.

Immunohistochemistry: Reported to detect Tie2/TEK in frozen, Triton-treated sections.
This antibody is not suitable for paraffin-embedded sections.
This Anti-Tie2/TEK Antibody, clone Ab33 is validated for use in WB, IP, IH for the detection of Tie2/TEK.

Qualité

Evaluated by western blot on RIPA lysates from HUVEC cells.

Western Blot Analysis: 0.2-1 μg/mL of this antibody detected Tie2/TEK in RIPA lysates from HUVEC cells.

Description de la cible

145 kDa

Forme physique

Format: Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide before the addition of glycerol to 30%. Liquid at -20°C.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Increased Expression of Angiopoietins and Tie2 in the Lungs of Chronic Asthmatic Mice.
Makinde TO, Agrawal DK
American Journal of Respiratory Cell and Molecular Biology null
Haixia Gong et al.
Scientific reports, 7, 42127-42127 (2017-02-16)
Human endothelial cells (ECs) are widely used to study mechanisms of angiogenesis, inflammation, and endothelial permeability. Targeted gene disruption induced by Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR-Associated Protein 9 (Cas9) nuclease gene editing is potentially an important tool for
Antiangiogenic gene therapy targeting the endothelium-specific receptor tyrosine kinase Tie2.
Lin, P, et al.
Proceedings of the National Academy of Sciences of the USA, 95, 8829-8834 (1998)
Mary Anna Venneri et al.
Blood, 109(12), 5276-5285 (2007-03-01)
Tumor-infiltrating myeloid cells, including tumor-associated macrophages (TAMs), have been implicated in tumor progression. We recently described a lineage of mouse monocytes characterized by expression of the Tie2 angiopoietin receptor and required for the vascularization and growth of several tumor models.
Daisuke Sakai et al.
JOR spine, 1(2), e1018-e1018 (2018-06-27)
Recently, Tie2/TEK receptor tyrosine kinase (Tie2 or syn. angiopoietin-1 receptor) positive nucleus pulposus progenitor cells were detected in human, cattle, and mouse. These cells show remarkable multilineage differentiation capacity and direct correlation with intervertebral disc (IVD) degeneration and are therefore
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