05-1406
Anti-Phospholipase A2 Antibody, clone CH-7
clone CH-7, from mouse
Synonyme(s) :
Phospholipase A2
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified antibody
Type de produit anticorps
primary antibodies
Clone
CH-7, monoclonal
Espèces réactives
rat, human, mouse
Technique(s)
ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
Isotype
IgG2b
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... PLA2G10(8399)
Description générale
Phospholipases hydrolyze phosphoglycerides to form smaller compounds which often serve as critical second messengers. Phospholipase A2 (PLA2) hydrolyzes phosphatidylcholine to produce lysophosphatidylcholine, a second messenger involved in cell proliferation, adhesion, T-cell activation, and smooth muscle cell contractility. PLA2 isoforms include membrane-associated, Ca2+-independent forms, cytosolic, Ca2+-dependent forms, and secretory forms. PLC catalyzes the hydrolysis of phosphatidylinositol 4,5-bisphosphate, to generate inositol (1,4,5) trisphosphate (IP3) and diacylglycerol (DAG), leading to Ca2+ release and PKC activation, respectively. At least four types of PLC have been identified. PLCβ, is activated by G-Protein-Coupled Receptors (GPCRs) and heterotrimeric G-Proteins. Mechanisms of PLCδ activation are unclear, but may also involve GPCRs. PLCγ, is activated by receptor tyrosine kinases, and appears essential for signaling by the PDGF Receptor. PLCε is a Guanine Nucleotide Exchange factor (GEF) for the small G-Protein Ras. PLD activity is up-regulated when cells are stimulated by hormones, growth factors or other extracellular signals. Two distinct PLDs, PLD1 and PLD2, have been identified. PLD hydrolyzes phosphatidylcholine to release phosphatidic acid, which is associated with a number of cellular responses including regulated secretion, mitogenesis and cytoskeletal changes.
Spécificité
This antibody recognizes PLA2.
Immunogène
Recombinant cPLA2
Application
Anti-Phospholipase A2 Antibody, clone CH-7 detects level of Phospholipase A2 & has been published & validated for use in WB, ELISA, IH(P).
ELISA: A previous lot of this antibody was used in ELISA.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry(paraffin): Representative images from a previous lot.
Optimal Staining of PLA2 Monoclonal Antibody: Colon Cancer
Western Blot: A previous lot of this antibody was used at 1:500-1:1000; antibody recognizes all isoforms, the cPLA. Alpha (85 kDa) typically migrates as 100-110 kDa on reducing westerns; beta migrates as 114 kDa; antibody will also recognize the sPLA at approximately 14-20 kDa.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry(paraffin): Representative images from a previous lot.
Optimal Staining of PLA2 Monoclonal Antibody: Colon Cancer
Western Blot: A previous lot of this antibody was used at 1:500-1:1000; antibody recognizes all isoforms, the cPLA. Alpha (85 kDa) typically migrates as 100-110 kDa on reducing westerns; beta migrates as 114 kDa; antibody will also recognize the sPLA at approximately 14-20 kDa.
Research Category
Signaling
Signaling
Research Sub Category
Lipid Signaling
Lipid Signaling
Qualité
Routinely evaluated by Western Blot on RAW 264 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected PLA2 on 10 μg of RAW 264 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected PLA2 on 10 μg of RAW 264 lysates.
Description de la cible
Alpha (85 kDa) typically migrates as 100-110 kDa on reducing westerns; beta migrates as 114 kDa; antibody will also recognize the sPLA at approximately 14-20 kDa
Liaison
Replaces: MAB1668
Forme physique
Format: Purified
Protein A purified
Purified mouse monoclonal IgG2b in buffer containing 20 mM sodium phosphate, 250 mM sodium chloride, 0.1% sodium azide, pH. 7.6.
Stockage et stabilité
Stable at 2-8°C for 1 year from date of receipt.
Remarque sur l'analyse
Control
RAW264 Cell Lysate
RAW264 Cell Lysate
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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S Martin et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 21(20), 8119-8128 (2001-10-06)
The node of Ranvier is a complex macromolecular assembly of ion channels and other proteins that is specialized for the rapid propagation of the action potential. A full understanding of the processes responsible for the assembly and maintenance of the
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