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790310P

Avanti

Transfection Reagent I

Avanti Research™ - A Croda Brand 790310P, powder

Synonyme(s) :

Transfection Reagent I, Transfection Reagent I, contains DOTAP:DOPE (1:1 w/w)

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About This Item

Code UNSPSC :
12352211
Nomenclature NACRES :
NA.25

Forme

powder

Conditionnement

pkg of 1 × 10 mg (790310P-10mg)
pkg of 2 × 100 mg (790310P-200mg)
pkg of 5 × 100 mg (790310P-500mg)
pkg of 1 × 25 mg (790310P-25mg)

Fabricant/nom de marque

Avanti Research™ - A Croda Brand 790310P

Application(s)

advanced drug delivery

Type de lipide

cationic lipids
transfection

Conditions d'expédition

dry ice

Température de stockage

−20°C

Catégories apparentées

Description générale

Transfection Reagent I comprises the cationic lipid 1,2-dioleoyloxypropyl)-N,N,N-trimethylammonium chloride (DOTAP) and the neutral lipid DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine. DOTAP is a surfactant with double chain quaternary ammonium. The DOTAP/DOPE liposome takes lamellar and inverted hexagonal structures.

Application

Transfection Reagent I has been used in the preparation of liposomes along with cholesterol for fluorescence-based live-cell imaging studies in the mouse embryonic fibroblasts cells. It may be used for the transfection of DNAzyme (Dz13) into veins.

Actions biochimiques/physiologiques

Transfection Reagent I is used in large unilamellar cationic vesicles (LUVs) preparation and lipoplex generation. It improves transfection efficiency. DOTAP/DOPE (1,2-dioleoyloxypropyl)-N,N,N-trimethylammonium chloride /(1,2-dioleoyl-sn-glycero-3-phosphoethanolamine) is safe transfection agent for human administration. It inhibits the blood vessel thickening and is a promising candidate for use in coronary artery bypass grafting (CABG).

Conditionnement

5 mL Clear Glass Sealed Ampule (790310P-10mg)
5 mL Clear Glass Sealed Ampule (790310P-200mg)
5 mL Clear Glass Sealed Ampule (790310P-25mg)
5 mL Clear Glass Sealed Ampule (790310P-500mg)

Informations légales

Avanti Research is a trademark of Avanti Polar Lipids, LLC

Code de la classe de stockage

11 - Combustible Solids

Point d'éclair (°F)

No data available

Point d'éclair (°C)

No data available

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Autophagy and formation of tubulovesicular autophagosomes provide a barrier against nonviral gene delivery
Roberts R, et al.
Autophagy, 9(5), 667-682 (2013)
Inhibition of vein graft stenosis with a c-jun targeting DNAzyme in a cationic liposomal formulation containing 1, 2-dioleoyl-3-trimethylammonium propane (DOTAP)/1, 2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE)
Li Y, et al.
International Journal of Cardiology, 168(4), 3659-3664 (2013)
Yue Li et al.
International journal of cardiology, 168(4), 3659-3664 (2013-07-28)
Coronary artery bypass grafting (CABG) is among the most commonly performed heart surgical procedures. Saphenous vein graft failure due to stenosis impedes the longer-term success of CABG. A key cellular event in the process of vein graft stenosis is smooth
Bieong-Kil Kim et al.
Biochimica et biophysica acta, 1848(10 Pt A), 1996-2001 (2015-06-27)
The effects of lipid compositions on their physicochemical properties and transfection efficiencies were investigated. Four liposome formulations with different 1,2-dioleoyl-3-trimethylammoniumpropane (DOTAP) to dioleoylphosphatidylethanolamine (DOPE) weight ratios were investigated, that is, weight ratios 1:0 (T1P0), 3:1 (T3P1), 1:1 (T1P1), and 1:3
DOTAP/DOPE and DC-Chol/DOPE lipoplexes for gene delivery: zeta potential measurements and electron spin resonance spectra
Ciani L, et al.
Biochimica et Biophysica Acta - Biomembranes, 1664(1), 70-79 (2004)
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