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906581

DLAM-A^βV^proS-¹³CH₃ Methyl Labeling Kit

Name: DLAM-AβVproS-13CH3 Methyl Labeling Kit
Brand: ALDRICH

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About This Item

Code UNSPSC :

12352200

Nomenclature NACRES :

NA.12

Technique(s)

bio NMR: suitable

Niveau de qualité

200

Conditions d'expédition

dry ice

Température de stockage

−70°C

Catégories apparentées

Isotopes stables

Description générale

DLAM-A^βV^proS-¹³CH₃kit has ¹³CH₃isotopomer precursors and contains protocol instructions for creation of isotopically-labeled proteins.

Application

DLAM-A^βV^proS-¹³CH₃ is an optimized kit to produce proteins with ¹³CH₃ isotopomer on β-methyl alanine and valine residues. This kit has been tested with protein isotopic labeling in E. coli. It is used in ¹H-¹³C methyl TROSY approach to study the interactions and dynamics in very large protein complexes. For protein-protein complexes spectral complexity is reduced using differentially labeled samples.

For detection of long-range nOes or for the NMR study of large proteins

Conditionnement

This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.

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A new labeling method for methyl transverse relaxation-optimized spectroscopy NMR spectra of alanine residues.

Rivka L Isaacson et al.

Journal of the American Chemical Society, 129(50), 15428-15429 (2007-11-29)

Characterizing Protein-Protein Interactions Using Solution NMR Spectroscopy.

Jose Luis Ortega-Roldan et al.

Methods in molecular biology (Clifton, N.J.), 1764, 73-85 (2018-04-02)

In this chapter, we describe how NMR chemical shift titrations can be used to study the interaction between two proteins with emphasis on mapping the interface of the complex and determining the binding affinity from a quantitative analysis of the

Methyl groups as NMR probes for biomolecular interactions.

Silke Wiesner et al.

Current opinion in structural biology, 35, 60-67 (2015-09-26)

Intermolecular interactions are indispensible for biological function. Here we discuss how novel NMR techniques can provide unique insights into the assembly, dynamics and regulation of biomolecular complexes. We focus on applications that exploit the methyl TROSY effect and show that

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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