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G005394

HPLC Analysis of Furocoumarins on Ascentis® Express RP-Amide

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis Express RP-Amide, 10 cm x 4.6 mm I.D., 2.7 μm particles (53929-U)
mobile phase: [A] water; [B] methanol; (40:60, A:B)
flow rate: 1.0 mL/min
pressure: 2843 psi (196 bar)
column temp.: 35 °C
detector: UV, 254 nm
injection: 5 μL
sample: 25 μg/mL in water:methanol (90:10)

suitability

application for HPLC

application(s)

vitamins, nutraceuticals, and natural products

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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