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Y1251

Millipore

Yeast Nitrogen Base Without Amino Acids and Ammonium Sulfate

suitable for microbiology, NutriSelect® Basic

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About This Item

UNSPSC Code:
41171606
NACRES:
NA.85

sterility

non-sterile

form

powder

packaging

poly bottle of 1 kg
poly bottle of 500 g

manufacturer/tradename

NutriSelect® Basic

solubility

water: soluble 67 mg/mL

application(s)

clinical testing
food and beverages
life science and biopharma
microbiology

suitability

Candida spp.
Pichia spp.
Saccharomyces spp.
Zygosaccharomyces spp.
molds
yeasts

General description

Yeast Nitrogen Base Without Amino Acids and Ammonium Sulfate is recommended to use for classifying yeasts based on their carbon and nitrogen requirements. The medium contains all essential nutrients required for the growth of yeasts except amino acids, histidine, methionine and tryptophan, and also ammonium sulphate. The addition of nitrogen and carbon sources is required as described in the Manual of Clinical Microbiology

Application

Yeast Nitrogen Base Without Amino Acids and Ammonium Sulfate is used in classifying yeasts based on carbon and nitrogen requirements. It contains all essential nutrients required for the growth of yeasts except amino acids, nitrogen and carbohydrate. The addition of nitrogen and carbon sources is required.

Components

Ingredients
Biotin, 2 μg/L
Calcium pantothenate, 400 μg/L
Folic acid, 2 μg/L
Niacin, 400 μg/L
p-Aminobenzoic acid, 200 μg/L
Pyridoxine HCl, 400 μg/L
Riboflavin, 200 μg/L
Thiamine HCl, 400 μg/L
Inositol, 2 mg/L
Boric acid, 500 μg/L
Copper sulfate, 40 μg/L
Potassium iodide, 100 μg/L
Ferric chloride, 200 μg/L
Manganese sulfate, 400 μg/L
Sodium molybdate, 200 μg/L
Zinc sulfate, 400 μg/L
Potassium phosphate monobasic, 1 g/L
Magnesium sulfate, 0.5 g/L
Sodium chloride, 0.1 g/L
Calcium chloride, 0.1 g/L

Preparation Note

  • Prepare a 10× stock solution by suspending 1.7 g of yeast nitrogen base without amino acids and ammonium sulfate in 100 ml of cold distilled water. Include desired nitrogen and carbon sources.
  • Warm if necessary to solubilize and sterilize by filtration.
  • Store 10× stock solution at 2-8°C. To use, dilute 1:10 with sterile distilled water under aspetic conditions.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit Components Only

Product No.
Description
  • KH2PO4 1 g/L
  • MgSO4 .5 g/L
  • NaCl .1 g/L
  • CaCl2 .1 g/L
  • Inositol 2 mg/L

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Owen W Ryan et al.
eLife, 3 (2014-08-21)
The directed evolution of biomolecules to improve or change their activity is central to many engineering and synthetic biology efforts. However, selecting improved variants from gene libraries in living cells requires plasmid expression systems that suffer from variable copy number
Yuping Lin et al.
Biotechnology for biofuels, 7(1), 126-126 (2014-12-02)
Saccharomyces cerevisiae, a key organism used for the manufacture of renewable fuels and chemicals, has been engineered to utilize non-native sugars derived from plant cell walls, such as cellobiose and xylose. However, the rates and efficiencies of these non-native sugar
Sviatlana Shashkova et al.
Bio-protocol, 8(2), e2710-e2710 (2018-02-13)
Single-molecule fluorescence microscopy enables unrivaled sub-cellular quantitation of genomically encoded fusions of native proteins with fluorescent protein reporters. Fluorescent proteins must undergo in vivo maturation after expression before they become photoactive. Maturation effects must be quantified during single-molecule analysis. Here
Fabiano de Assis Gontijo et al.
PloS one, 12(5), e0177461-e0177461 (2017-05-26)
In order to survive and cause disease, microbial pathogens must be able to proliferate at the temperature of their infected host. We identified novel microbial features associated with thermotolerance in the opportunistic fungal pathogen Cryptococcus neoformans using a random insertional
Alexander Beck et al.
Frontiers in bioengineering and biotechnology, 8, 555280-555280 (2020-11-17)
Fungi of the Ustilaginaceae family are a promising source for many biotechnologically relevant products. Among these, mannosylerythritol lipid (MEL) biosurfactants have drawn a special interested over the last decades due to their manifold application possibilities. Nevertheless, there is still a
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