SRP2011

TFIIH, p62 subunit human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

• Synonym(s): BTF2, TFB1, TFIIH
• UNSPSC Code: 12352200
• NACRES: NA.26

Properties

• biological source: human
• recombinant: expressed in E. coli
• Assay: ≥70% (SDS-PAGE)
• form: frozen liquid
• mol wt: ~64.4 kDa
• packaging: pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• concentration: 750 μg/mL
• color: clear colorless
• NCBI accession no.: NM_005316
• UniProt accession no.: P32780
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... GTF2H1(2965)

Description

Biochem/physiol Actions

TFIIH, a multi-subunit complex is involved in several biological fundamental mechanisms of the cell: transcription, nucleotide excision repair and cell cycle regulation. p62 is one of the six subunits that constitutes the core of TFIIH. Analysis of the expression of the p62 gene reveals an over-expression in testis tissue. This subunit of TFIIH participates in a variety of protein-protein interactions. For example, Rb competes with TBP and p62 for binding to E2F thus repressing E2F-mediated trans-activation; herpes simplex virus VP16 and human p53 directly interact with the p62 subunit of TFIIH. In addition, TFIIH, via p62 phosphorylation is the major target for mitotic inactivation of transcription.

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable