Skip to Content
Merck
All Photos(1)

Key Documents

SRP0217

Sigma-Aldrich

SHP-2 Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

Synonym(s):

Noonan syndrome 1, PTP-1D, PTP2C, PTPN11, SHP2

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352200
NACRES:
NA.32
Pricing and availability is not currently available.

biological source

human

recombinant

expressed in E. coli

Assay

≥90% (SDS-PAGE)

form

aqueous solution

mol wt

59.5 kDa

packaging

pkg of 20 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

0.73 mg/mL

NCBI accession no.

NM_002834

UniProt accession no.

Q06124

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PTPN11(5781)

General description

Protein tyrosine phosphatase, non-receptor type 11 (PTPN11), also known as SHP-2, is a scaffolding protein. It possesses two SH2 domains at the amino terminal, a protein tyrosine phosphatase domain and carboxy-terminal region. The gene encoding SHP-2 is localized on human chromosome 12q24.13.[1][2][3]

Application

Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.
Human active SHP-2 has been used to study the effects of α-lipoic acid and dihydrolipoic acid on the activity of SHP-2 enzyme.[4]

Biochem/physiol Actions

Protein tyrosine phosphatase, non-receptor type 11 (PTPN11) or SHP-2 is involved in signal transduction pathways downstream of growth receptors. It acts as a proto-oncogene. SHP-2 leads to sustained RAS activation by dephosphorylating the RAS-GTPase-activating protein binding site on Grb2-associated binder 1 (Gab1). This protein also nucleates multiple signaling proteins and thus, acts as an adaptor protein. Mutations in the SHP-2 gene have been associated with Noonan syndrome.[5][1][2]

Unit Definition

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Other Notes

Amino acids : 224-529

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
3002230807
30022090608
3002290608

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Shp2 Plays a Critical Role in IL-6-Induced EMT in Breast Cancer Cells.
Sun X
International Journal of Molecular Sciences, 18(2) (2017)
Hotspots in PTPN11 Gene Among Indian Children With Noonan Syndrome.
Narayanan DL
Indian Pediatrics, 54(8), 638-643 (2017)
Lipoic Acid Decreases the Viability of Breast Cancer Cells and Activity of PTP1B and SHP2
Anticancer Research, 37(6) (2017)
SHP2 is induced by the HBx-NF-?B pathway and contributes to fibrosis during human early hepatocellular carcinoma development.
Kang HJ
Oncotarget, 8(16) (2017)
Naphak Modhiran et al.
Science translational medicine, 7(304), 304ra142-304ra142 (2015-09-12)
Complications arising from dengue virus infection include potentially fatal vascular leak, and severe disease has been linked with excessive immune cell activation. An understanding of the triggers of this activation is critical for the development of appropriately targeted disease control
View All Related Papers

Questions

  1. What is the difference between products SRP0217 and SRP5072? I cannot find Data Sheet for product SRP0217. Is the handling of the product SRP0217 the same as for the product SRP5072? What is the sequence of the product SRP0217 (SHP-2 Active human)?

    1 answer

    1. The products are similar in many respects but differ in storage buffer, sequence, purity, and molecular weight. Both are active and are n-terminal GST tagged.

      SPR0217
      In Tris buffer solution w/50% glycerol, pH 8
      Amino acids 224 - 529
      >90% purity
      MW 59.5 kDa

      SRP5072
      In MOPS buffer solution w/30% glycerol, pH 7.5
      Amino acids 246 - 593
      >70% purity
      MW ~69 kDa

      See the link below to the UniProt page for further information on this enzyme:
      https://www.uniprot.org/uniprotkb/Q06124/entry

      Helpful?

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service