N1665
5′-Nucleotidase human
recombinant, expressed in CHO cells, vial of 6-12 μg
Synonym(s):
CD73, NT5E, ecto-5′-nucleotidase
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Recommended Products
recombinant
expressed in CHO cells
Quality Level
description
contains a C-terminal 6-His tag
Assay
≥90% (SDS-PAGE)
form
solution
enzyme activity
≥15 units/mg protein
mol wt
~61 kDa by SDS-PAGE (reducing)
packaging
vial of 6-12 μg
impurities
≤1.0 EU/μg endotoxin
NCBI accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... NT5E(4907)
General description
5′-Nucleotidase is an extracellular enzyme found in a wide variety of mammalian cells. It helps to accelerate the hydrolysis reaction of phosphate group from 5′ nucleotides, resulting in corresponding nucleosides.
Biochem/physiol Actions
5′-Nucleotidase has various clinical significances. It is a key molecule in the regulation of cancer cells proliferation, migration and invasion in vitro tumor angiogenesis, and tumor immune escape in vivo. Due to this important role, the enzyme is a potential target for cancer research. It is also involved in salvage of extracellular nucleotides and plays a key role in the control of tissue homeostasis.
5′-nucleotidase, also known as CD73, is an extracellular enzyme that converts nucleoside-5′−monophosphates to nucleosides with a substrate preference of AMP. Native 5′-nucleotidase is a GPI-anchored protein whose exporession is upregulated by hypoxia. 5′-nucleotidase has many functions in vivo including the generation of extracellular adenosine.
Unit Definition
One unit will hydrolyze 1.0 μmole of inorganic phosphorus from adenosine 5′-monophosphate per min at pH 9.0 at 37 °C.
Physical form
Supplied as a solution containing Tris, NaCl, CaCl2, and 20% glycerol.
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Isolation of a plasma-membrane subfraction from rat liver containing an insulin-sensitive cyclic-AMP phosphodiesterase.
P D House et al.
European journal of biochemistry, 24(3), 429-437 (1972-01-21)
Zhao-wei Gao et al.
BioMed research international, 2014, 460654-460654 (2014-08-16)
Purinergic signaling has emerged as an important player in cancer progression and is regulated by a series of nucleotidases. Among the enzyme cascade, CD73, which catelyzes AMP breakdown to adenosine, has been found to be overexpressed in many types of
Anna Wilk et al.
Scientific reports, 10(1), 651-651 (2020-01-22)
Changes in nicotinamide adenine dinucleotide (NAD+) levels that compromise mitochondrial function trigger release of DNA damaging reactive oxygen species. NAD+ levels also affect DNA repair capacity as NAD+ is a substrate for PARP-enzymes (mono/poly-ADP-ribosylation) and sirtuins (deacetylation). The ecto-5'-nucleotidase CD73
Paul A Beavis et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(36), 14711-14716 (2013-08-22)
CD73 inhibits antitumor immunity through the activation of adenosine receptors expressed on multiple immune subsets. CD73 also enhances tumor metastasis, although the nature of the immune subsets and adenosine receptor subtypes involved in this process are largely unknown. In this
5'-Nucleotidase: molecular structure and functional aspects.
H Zimmermann
The Biochemical journal, 285 ( Pt 2), 345-365 (1992-07-15)
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