M8883

4-Methylumbelliferyl phosphate

phosphatase substrate, fluorogenic, ≥98% (HPLC), powder

• Synonym(s): 4-Methylumbelliferyl-phosphoric acid
• Empirical Formula (Hill Notation): C₁₀H₉O₆P
• CAS Number: 3368-04-5
• Molecular Weight: 256.15
• Beilstein: 1687229
• EC Number: 222-137-7
• MDL number: MFCD00016969
• UNSPSC Code: 12352204
• PubChem Substance ID: 24897292
• NACRES: NA.32

Properties

• product name: 4-Methylumbelliferyl phosphate, phosphatase substrate
• description: phosphatase substrate
• Assay: ≥98% (HPLC)
• form: powder
• solubility: water: 25 mg/mL, clear to very slightly hazy, colorless
• fluorescence: λ_ex 319 nm; λ_em 384 nm (pH 9.1); λ_ex 360 nm; λ_em 449 nm (Reaction product)
• storage temp.: −20°C
• SMILES string: CC1=CC(=O)Oc2cc(OP(O)(O)=O)ccc12
• InChI: 1S/C10H9O6P/c1-6-4-10(11)15-9-5-7(2-3-8(6)9)16-17(12,13)14/h2-5H,1H3,(H2,12,13,14)
• InChI key: BCHIXGBGRHLSBE-UHFFFAOYSA-N

Description

Application

4-Methylumbelliferyl phosphate has been used as a substrate for alkaline phosphatase in the peptide-binding assay. It has also been used as a fluorogenic substrate in enzyme-linked immunosorbent assay (ELISA).

Biochem/physiol Actions

4-Methylumbelliferyl phosphate serves as a fluorogenic substrate for calmodulin-dependent protein phosphatase and in kinetic studies of alkaline phosphatase. It is a substrate for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures. 4-Methylumbelliferyl phosphate is seven times more sensitive than phenolphthalein monophosphate and 8-13 times more sensitive than with p-nitrophenyl phosphate when used in enzyme immunoassays for the detection of antibodies to human immunodeficiency viruses.

Substrates

Fluorogenic substrate for phosphatases.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves