I6527

Anti-Interleukin-4 Soluble Receptor antibody produced in goat

IgG fraction of antiserum, lyophilized powder

• Synonym(s): Anti-IL-4 sR
• MDL number: MFCD02096774
• UNSPSC Code: 51111800
• NACRES: NA.41

Properties

• biological source: goat
• Quality Level: 100
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: lyophilized powder
• species reactivity: human
• technique(s): indirect ELISA: suitable; neutralization: suitable; western blot: suitable
• UniProt accession no.: P24394
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... IL4R(3566)

Description

General description

Interleukin-4 (IL-4) is a T cell derived chemokine that stimulates the Th2 mediated immune response and proliferation of B cells. The effects of IL-4 are mediated by two types of receptors, type I receptor consisting of IL-4Rα and common γ chain and type II receptor composed of IL-4Rα and IL-13Rα1. The signalling stimulated by IL-4R leads to activation of JAK/STAT6 and IRS-mediated PI3K/Akt pathway. Through these pathways, IL-4 is responsible for endocytic activity of macrophages, chemotaxis of leukocytes in response to inflammation, angiogenesis and regulation of nitric oxide metabolism in macrophages. Anti-tumor effects of IL-4R signaling have been reported in cancers of breast, liver and renal cells
Anti-Interleukin-4 soluble receptor specifically recognizes human cell surface IL-4R.

Specificity

The antibody will neutralize human cell surface IL-4 receptor mediated-bioactivity.

Immunogen

recombinant human IL-4 soluble receptor (IL-4 sR), expressed in Sf 21 insect cells.

Application

Anti-Interleukin-4 soluble receptor antibody may be used for immunoblotting at a working concentration of 1-2 μg/ml. For ELISA, a working concentration of 0.5-1.0 μg/ml is recommended. The antibody is suitable for neutralization reactions at a working concentration of 5-10 μg/ml. This antibody was used for immunostaining of parasympathetic neurons from guinea pig and human neuroblastoma cells at a dilution of 1:200.

Physical form

Lyophilized from 51.5 μL of a 0.2μm filitered solution in PBS (pH7.4) with 5% trehalose.

Preparation Note

Purified using Protein G.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable