51120

Guanosine 5′-triphosphate sodium salt hydrate

≥90% (HPLC)

• Synonym(s): GTP, GTP-Na⁺ hydrate, 5′-GTP-Na₂
• Empirical Formula (Hill Notation): C₁₀H₁₆N₅O₁₄P₃ · xNa⁺ · yH₂O
• CAS Number: 36051-31-7
• Molecular Weight: 523.18 (anhydrous free acid basis)
• Beilstein: 4113439
• EC Number: 252-847-2
• MDL number: MFCD00149227
• UNSPSC Code: 41106305
• PubChem Substance ID: 57651277
• NACRES: NA.51

Properties

• Assay: ≥90% (HPLC)
• form: powder
• optical activity: [α]20/D −24±2°, c = 1% in 0.5 M Na₂HPO₄
• impurities: ≤3 mol/mol water (NMR)
• shipped in: wet ice
• storage temp.: −20°C
• SMILES string: O.O.[Na+].[Na+].NC1=Nc2c(ncn2[C@@H]3O[C@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)[C@@H](O)[C@H]3O)C(=O)N1
• InChI: 1S/C10H16N5O14P3.2Na.2H2O/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21;;;;/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18);;;2*1H2/q;2*+1;;/p-2/t3-,5-,6-,9-;;;;/m1..../s1
• InChI key: HPLNHYUZFVUXFJ-ZVQJTLEUSA-L

Description

General description

Guanosine 5′-triphosphate (GTP) is a purine trinucleotide. GTP is a substrate for a wide range of G-protein coupled receptor (GPCR) GTPases, as well as cell signaling and cycling-associated guanine nucleotide exchange factors (GEF). See product G3776 for RNA polymerase transcription and DNA or RNA biosynthesis applications.

Application

Guanosine 5′-triphosphate sodium salt hydrate has been used:

• as a component of the internal solution for astrocyte patch-clamp to monitor astrocyte calcium activity from mouse amygdala slices.
• in a solution for channel current recording in cell lines using a voltage-clamp technique.
• to prepare internal recording solution for injectoporation to deliver genes to mechanosensory hair cells.
• promoting the formation of G interface-mediated homodimers of human septin during the analysis of its oligomerization state by analytical size exclusion chromatography (SEC).

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves