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MAB2074Z

Sigma-Aldrich

Anti-Integrin αVβ6 Antibody, clone E7P6, azide free

clone E7P6, Chemicon®, from mouse

Synonym(s):

MAB2074

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

E7P6, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable

isotype

IgG1

suitability

not suitable for activity/function inhibition
not suitable for immunohistochemistry (Paraffin)

NCBI accession no.

NM_002210.2

UniProt accession no.

P06756

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ITGAV(3685)

Related Categories

Specificity

Monoclonal antibody MAB2074Z, raised by immunizing Balb/c mice with recombinant secreted human avb6, is useful for flow cytometry and is especially useful for immunocytochemistry. The antibody works well for immunohistochemistry of human tissue, especially under circumstances where upregulation of the receptor is being evaluated. The antibody does not stain formalin-fixed or paraffin-embedded tissues, and may not detect the low-level basal expression of alphavbeta6 in epithelia of healthy adults. This antibody does not recognize the alphaV subunit and does not react with alphaV integrins other than alphavbeta6. Because the antibody is not effective in western blot, it is unknown whether the epitope recognized by the antibody localizes to the alphavbeta6 heterodimer or to the beta6 subunit only.

Immunogen

Recombinant secreted human alphavbeta6.

Application

Immunohistochemistry (acetone fixation): Effective for human tissue, especially in circumstances where up-regulation of the receptor is being evaluated. The antibody does not stain formalin-fixed or paraffin-embedded tissues, and may not detect the low-level basal expression of alpavbeta6 in epithelia of healthy adults.

Immunocytochemistry: Effective for F6, HT-29, H441 cell lines (others not tested).

Flow cytometry: Effective for detection of human and monkey alphaVbeta6

Not effective for immunoprecipitation or western blot.

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Integrins
This Anti-Integrin αVβ6 Antibody, clone E7P6, azide free is validated for use in FC, IC, IH for the detection of Integrin αVβ6.

Physical form

Format: Purified
Sterile-filtered liquid at 1 mg/mL in PBS, containing no preservatives.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 12 months.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Christopher J Legge et al.
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, 48(9), 803-809 (2019-07-17)
Patients with oral squamous cell carcinoma currently experience a five-year survival rate of approximately 60% with conventional surgical, chemotherapy and radiotherapy treatments. Magnetic hyperthermia offers an alternative treatment method by utilising the heating properties of magnetic nanoparticles to produce thermal
Jiarong Wang et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 36(6), 2198-2216 (2015-08-19)
IL-1β creates persistent pulmonary inflammation accompanied by elevated transforming growth factor β (TGF-β levels and is associated with abnormal elastogenesis, which is observed in bronchopulmonary dysplasia (BPD). Although progress has been made in this field, the mechanisms underlying this process
Stephen Berryman et al.
Journal of virology, 79(13), 8519-8534 (2005-06-16)
We have shown that foot-and-mouth disease virus (FMDV) infection mediated by the integrin alphavbeta6 takes place through clathrin-dependent endocytosis but not caveolae or other endocytic pathways that depend on lipid rafts. Inhibition of clathrin-dependent endocytosis by sucrose treatment or expression
M R Morgan et al.
Oncogene, 30(12), 1422-1435 (2010-12-07)
Expression of the integrin αvβ6 is upregulated in a variety of carcinomas where it appears to be involved in malignant progression, although the biology of this integrin is not fully explored. We have generated oral carcinoma cells that express αvβ6
A peptide selected by biopanning identifies the integrin alphavbeta6 as a prognostic biomarker for nonsmall cell lung cancer.
Elayadi, AN; Samli, KN; Prudkin, L; Liu, YH; Bian, A; Xie, XJ; Wistuba, II; Roth et al.
Cancer Research null
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