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Key Documents

AB1785P

Sigma-Aldrich

Anti-Dopamine D3 Receptor Antibody, cytoplasmic domain

Chemicon®, from rabbit

Synonym(s):

D3R

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... DRD3(1814)

Specificity

Specific for Dopamine Receptor D3. Human Dopamine D3 receptor is a 400 aa, G-protein coupled and transmembrane receptor protein (Giros et al. 1990, 1991; Schmauss et al. 1993; Liu et al. 1994; Fu et al. 1995; Sokoloff et al. 1990; Livingstone et al. 1992). The immunogen peptide shows no significant sequence similarity with other dopamine receptors (D1, D2, D4 or D5).

Immunogen

A 19 amino acid peptide sequence from the human D3 receptor within the 3rd cytoplasmic domain.
Epitope: cytoplasmic domain

Application

Detect Dopamine D3 Receptor using this Anti-Dopamine D3 Receptor Antibody, cytoplasmic domain validated for use in ELISA, IH & WB.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Western blot: 1-10 μg/mL (Chemiluminescence technique) Approximately 100-400 μg of membrane protein/lane should be loaded to visualize the receptor band.

Immunohistochemistry: 1-20 μg/mL on paraformaldehyde fixed sections.

ELISA: 0.5-1.0 μg/mL (1 μg/mL immunogen peptide (Cat. Number AG224)/well)

Optimal working dilutions must be determined by the end user.

Physical form

Affinity purified immunoglobulin. Liquid in PBS, pH 7.5 with 0.1% BSA. No preservatives

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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