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C1104

Sigma-Aldrich

Monoclonal Anti-Caspase 7 antibody produced in rat

clone 11E4, purified immunoglobulin, buffered aqueous solution

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rat

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

11E4, monoclonal

form

buffered aqueous solution

mol wt

antigen (full length) 35 kDa
antigen (subunit) 20 kDa

species reactivity

human

technique(s)

immunoprecipitation (IP): suitable
microarray: suitable
western blot: 2-4 μg/mL using a whole extract of cultured human acute T cell leukemia Jurkat cells

isotype

IgG2a

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CASP7(840)

General description

Caspase-7 is a apoptosis-related cysteine peptidase, commonly known as CASP7. It is a human protein encoded by the CASP7 gene. CASP7 is a member of the caspase (cysteine aspartate protease) family of proteins and is an executioner protein of apoptosis.

Immunogen

recombinant p20 subunit of human caspase-7.

Application

Monoclonal Anti-Caspase 7 antibody produced in rat is suitable for the following applications:
  • Immunoprecipitation
  • Microarray
  • Western blotting at a concentration of 2-4μg/mL using a whole extract of cultured human acute T cell leukemia Jurkat cells.

Biochem/physiol Actions

Caspase-3 and -7 plays a central role in the execution-phase of cell apoptosis. The gene for caspase 7 (also known as Mch3, CMH-1 and ICELAP3) encodes a “short” prodomain protein product of 35 kDa that belongs to the Ced3/ICE family of cysteine proteases. Caspase 7 undergoes acleavage upon activation by caspase-3, -9 and -10, and granzyme B, leading to p20 and p12 subunits. It is an important intracellular effector of granzyme B-mediated apoptosis and cytotoxic Tlymphocyte- induced cell killing in vivo. Monoclonal antibodies reacting specifically with caspase 7 are useful tools for the study of protease networks involved in development and regulation and governing the life and death of cells and tissues.

Preparation Note

The antibody is purified from culture supernatant of hybridoma cells grown in a bioreactor.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A M Chinnaiyan et al.
Current biology : CB, 6(7), 897-899 (1996-07-01)
Cytotoxic T lymphocytes (CTLs) and natural killers (NK) cells provide immune surveillance against viruses and neoplasms, and play a central role in the pathogenesis of autoimmune disease, AIDS and graft rejection. Thus, it is important to understand the precise molecular
Witold A Witkowski et al.
Protein science : a publication of the Protein Society, 20(8), 1421-1431 (2011-06-16)
Caspases are a powerful class of cysteine proteases. Introduction of activated caspases in healthy or cancerous cells results in induction of apoptotic cell death. In this study, we have designed and characterized a version of caspase-7 that can be inactivated
E Svandova et al.
Cell death & disease, 5, e1366-e1366 (2014-08-15)
Caspase-3 and -7 are generally known for their central role in the execution of apoptosis. However, their function is not limited to apoptosis and under specific conditions activation has been linked to proliferation or differentiation of specialised cell types. In
Yin Guo et al.
The Journal of biological chemistry, 277(16), 13430-13437 (2002-02-08)
Caspase-2 is one of the earliest identified caspases, but the mechanism of caspase-2-induced apoptosis remains unknown. We show here that caspase-2 engages the mitochondria-dependent apoptotic pathway by inducing the release of cytochrome c (Cyt c) and other mitochondrial apoptogenic factors
J M Chandler et al.
The Journal of biological chemistry, 273(18), 10815-10818 (1998-06-06)
Caspases plays a key role in the execution phase of apoptosis. "Initiator" caspases, such as caspase-8, activate "effector" caspases, such as caspase-3 and -7, which subsequently cleave cellular substrates thereby precipitating the dramatic morphological changes of apoptosis. Following treatment of

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