C0678
Monoclonal Anti-HNK-1/N-CAM (CD57) antibody produced in mouse
clone VC1.1, ascites fluid, buffered aqueous solution
Synonym(s):
Monoclonal Anti-HNK-1, Anti-CD57, Anti-N-CAM
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About This Item
Recommended Products
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
VC1.1, monoclonal
form
buffered aqueous solution
contains
15 mM sodium azide
species reactivity
mouse, rat, feline, human
technique(s)
western blot: 1:4,000 using cerebral cortex extract
isotype
IgM
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... B3GAT1(27087)
mouse ... B3gat1(76898)
rat ... B3gat1(117108)
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General description
Monoclonal Anti-HNK-1/N-CAM (CD57) (mouse IgM isotype) is derived from the VC1.1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Human natural killer cell -1 (HNK-1), also referred as B3GAT1, NK1, CD57, LEU7, NK-1, GLCATP and GLCUATP, is mapped to human chromosome 11q25. The encoded protein belongs to the glucuronyltransferase gene family. Neural cell-adhesion molecule (N-CAM) is expressed on all neuroepithelial cells from the time of neural induction in the early embryo. It is known that the sulfated carbohydrate HNK-1 epitope is carried by several surface molecules including a proportion of N-CAM isoforms.
Specificity
Recognizes the CD57/HNK-1 human myeloid cell associated surface glycoprotein. The epitope recognized is an N-linked carbohydrate which is present in a variety of glycoproteins and in some glycolipids. It is resistant to formalin fixation and paraffin embedding. VC1.1 antibody and the HNK-1 (Leu7) antibody inhibit the binding of each other. The antibody recognizes myelin associated glycoprotein in some species and a high molecular weight chondroitin sulphate proteoglycan.
5th Workshop: code no. NK 67
5th Workshop: code no. NK 67
Immunogen
A homogenate of cat primary visual cortex (area 17)
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Immunohistochemistry (1 paper)
Monoclonal Anti-HNK-1/N-CAM (CD57) antibody produced in mouse has been used:
- as primary antibody for western blot analysis
- immunocytochemistry
- immunocytochemical
- immunohistological staining
Biochem/physiol Actions
HNK1 (human natural killer-1) is referred as B3GAT1, NK1, CD57, LEU7, NK-1, GLCATP and GLCUATP. It is a member of the glucuronyltransferase gene family. Human natural killer-1 (HNK1) carbohydrate epitope is expressed on various cell adhesion molecules in the nervous system and plays a role in cell-cell and cell-substrate interactions. HNK-1 epitope stabilizes GluR2 on neuronal surface membranes and regulates cell surface stability of GluR2 by modulating the interaction with N-cadherin. It is also plays a role in cell migration and synaptic plasticity. HNK-1 epitope has been associated with the risk of metastasis. It is observed to be highly expressed in melanoma cell lines.
Target description
CD57/HNK-1 is expressed on a subpopulation of 15-20% of peripheral blood mononuclear cells, about 60% of NK active cells and on a subset of T cells.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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